{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T16:29:49Z","timestamp":1764001789742},"reference-count":33,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2011,5,15]],"date-time":"2011-05-15T00:00:00Z","timestamp":1305417600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Orientia tsutsugamushi<\/jats:named-content>is the etiological agent of scrub typhus, a mite-borne, febrile illness that occurs in the Asia-Pacific region. We conducted strain characterization of<jats:named-content content-type=\"genus-species\">O. tsutsugamushi<\/jats:named-content>isolates from chiggers obtained from rodents based the nucleotide sequence of the 56-kDa outer membrane protein gene. With the use of PCR, a total of 68 DNA sequences of 56-kDa antigen genes were amplified. Phylogenetic analysis revealed that there were at least six definable clusters among the 68 isolates: 37% Karp-related strains (25\/68), 27% TA763 strains (18\/68), 12% JG-related strains (8\/68), 19% Kato-related strains (13\/68), 4% divergent strains (3\/68), and 1% representing a Gilliam prototype strain (1\/68). Overall, the<jats:named-content content-type=\"genus-species\">O. tsutsugamushi<\/jats:named-content>genotypes exhibited a high degree of diversity, similar to that seen in strains from the rest of the areas where scrub typhus is endemic. Moreover, the 56-kDa protein sequence similarity between<jats:named-content content-type=\"genus-species\">O. tsutsugamushi<\/jats:named-content>isolates from mites and those from human patients (H. Y. Lu et al., Am. J. Trop. Med. Hyg. 83:658-663, 2010) were striking, thus highlighting potential risk factors for this emerging zoonotic disease.<\/jats:p>","DOI":"10.1128\/aem.02796-10","type":"journal-article","created":{"date-parts":[[2011,3,26]],"date-time":"2011-03-26T03:53:55Z","timestamp":1301111635000},"page":"3398-3405","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Genetic Typing, Based on the 56-Kilodalton Type-Specific Antigen Gene, of Orientia tsutsugamushi Strains Isolated from Chiggers Collected from Wild-Caught Rodents in Taiwan"],"prefix":"10.1128","volume":"77","author":[{"given":"Pey-Ru","family":"Lin","sequence":"first","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-Ping","family":"Tsai","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei-Yi","family":"Tsui","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Hui","family":"Weng","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Der","family":"Kuo","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hung-Chi","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuo-Ching","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dar-Der","family":"Ji","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Der-Ming","family":"Chu","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Tssann","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2007.00375.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.76.553"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.8.5039-5047.2005"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1355.006"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.6.1637-1640.1993"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1974.23.679"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1996.54.647"},{"key":"e_1_3_3_9_2","first-page":"394","article-title":"Evaluation of an immunoglobulin M capture enzyme-linked immunosorbent assay for diagnosis of Orientia tsutsugamushi infection","volume":"10","author":"Jang W. 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