{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:19:52Z","timestamp":1790745592683,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2017,2,15]],"date-time":"2017-02-15T00:00:00Z","timestamp":1487116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Australian National Health and Medical Research Council"},{"DOI":"10.13039\/100000774","name":"DOD | Defense Threat Reduction Agency","doi-asserted-by":"publisher","award":["HDTRA1-12-C-0066"],"award-info":[{"award-number":["HDTRA1-12-C-0066"]}],"id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000774","name":"DOD | Defense Threat Reduction Agency","doi-asserted-by":"publisher","award":["HSHQDC-10-C-00149"],"award-info":[{"award-number":["HSHQDC-10-C-00149"]}],"id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000180","name":"U.S. Department of Homeland Security","doi-asserted-by":"publisher","award":["HSHQDC-10-C-00135"],"award-info":[{"award-number":["HSHQDC-10-C-00135"]}],"id":[{"id":"10.13039\/100000180","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000180","name":"U.S. Department of Homeland Security","doi-asserted-by":"publisher","award":["HSHQDC-10-C-00149"],"award-info":[{"award-number":["HSHQDC-10-C-00149"]}],"id":[{"id":"10.13039\/100000180","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"ABSTRACT<\/jats:title>\n \n During routine screening for\n Burkholderia pseudomallei<\/jats:named-content>\n from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43\n T<\/jats:sup>\n , MSMB121, and MSMB122) with a similar morphology and biochemical pattern to\n B. pseudomallei<\/jats:named-content>\n and\n B. thailandensis<\/jats:named-content>\n were coisolated with\n B. pseudomallei<\/jats:named-content>\n on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from\n B. pseudomallei<\/jats:named-content>\n and\n B. thailandensis<\/jats:named-content>\n , they were subjected to a series of phenotypic and molecular analyses. Biochemical and fatty acid methyl ester analysis was unable to distinguish\n B. humptydooensis<\/jats:named-content>\n sp. nov. from closely related species. With matrix-assisted laser desorption ionization\u2013time of flight analysis, all isolates grouped together in a cluster separate from other\n Burkholderia<\/jats:named-content>\n spp. 16S rRNA and\n recA<\/jats:italic>\n sequence analyses demonstrated phylogenetic placement for\n B. humptydooensis<\/jats:named-content>\n sp. nov. in a novel clade within the\n B. pseudomallei<\/jats:named-content>\n group. Multilocus sequence typing (MLST) analysis of the three isolates in comparison with MLST data from 3,340\n B. pseudomallei<\/jats:named-content>\n strains and related taxa revealed a new sequence type (ST318). Genome-to-genome distance calculations and the average nucleotide identity of all isolates to both\n B. thailandensis<\/jats:named-content>\n and\n B. pseudomallei<\/jats:named-content>\n , based on whole-genome sequences, also confirmed\n B. humptydooensis<\/jats:named-content>\n sp. nov. as a novel\n Burkholderia<\/jats:named-content>\n species within the\n B. pseudomallei<\/jats:named-content>\n complex. Molecular analyses clearly demonstrated that strains MSMB43\n T<\/jats:sup>\n , MSMB121, and MSMB122 belong to a novel\n Burkholderia<\/jats:named-content>\n species for which the name\n Burkholderia humptydooensis<\/jats:named-content>\n sp. nov. is proposed, with the type strain MSMB43\n T<\/jats:sup>\n (American Type Culture Collection BAA-2767; Belgian Co-ordinated Collections of Microorganisms LMG 29471; DDBJ accession numbers\n CP013380<\/jats:ext-link>\n to\n CP013382<\/jats:ext-link>\n ).\n <\/jats:p>\n \n IMPORTANCE<\/jats:bold>\n Burkholderia pseudomallei<\/jats:named-content>\n is a soil-dwelling bacterium and the causative agent of melioidosis. The genus\n Burkholderia<\/jats:named-content>\n consists of a diverse group of species, with the closest relatives of\n B. pseudomallei<\/jats:named-content>\n referred to as the\n B. pseudomallei<\/jats:named-content>\n complex. A proposed novel species,\n B. humptydooensis<\/jats:named-content>\n sp. nov., was isolated from a bore water sample from the Northern Territory in Australia.\n B. humptydooensis<\/jats:named-content>\n sp. nov. is phylogenetically distinct from\n B. pseudomallei<\/jats:named-content>\n and other members of the\n B. pseudomallei<\/jats:named-content>\n complex, making it the fifth member of this important group of bacteria.\n <\/jats:p>","DOI":"10.1128\/aem.02802-16","type":"journal-article","created":{"date-parts":[[2016,12,23]],"date-time":"2016-12-23T17:51:06Z","timestamp":1482515466000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Burkholderia humptydooensis sp. nov., a New Species Related to Burkholderia thailandensis and the Fifth Member of the Burkholderia pseudomallei Complex"],"prefix":"10.1128","volume":"83","author":[{"given":"Apichai","family":"Tuanyok","sequence":"first","affiliation":[{"name":"Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Mayo","sequence":"additional","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, and Northern Territory Medical Program, Royal Darwin Hospital, Darwin, Northern Territory, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Scholz","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carina M.","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Allender","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirjam","family":"Kaestli","sequence":"additional","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, and Northern Territory Medical Program, Royal Darwin Hospital, Darwin, Northern Territory, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Ginther","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, 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