{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:49:45Z","timestamp":1791118185112,"version":"4.1.0"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n This is the first report to characterize the genotypes and subtypes of\n Cryptosporidium<\/jats:named-content>\n species infecting a geographically isolated population of feral Soay sheep (\n Ovis aries<\/jats:named-content>\n ) on Hirta, St. Kilda, Scotland, during two distinct periods: (i) prior to a population crash and (ii) as host numbers increased.\n Cryptosporidium<\/jats:named-content>\n DNA was extracted by freeze-thawing of immunomagnetically separated (IMS) bead-oocyst complexes, and species were identified following nested-PCR-restriction fragment length polymorphism (RFLP)\/PCR sequencing at two\n Cryptosporidium<\/jats:named-content>\n 18S rRNA loci. Two hundred fifty-five samples were analyzed, and the prevalent\n Cryptosporidium<\/jats:named-content>\n species in single infections were identified as\n C. hominis<\/jats:named-content>\n (11.4% of all samples tested),\n C. parvum<\/jats:named-content>\n (9%),\n C. xiaoi<\/jats:named-content>\n (12.5%), and\n C. ubiquitum<\/jats:named-content>\n (6.7%).\n Cryptosporidium parvum<\/jats:named-content>\n was also present with other\n Cryptosporidium<\/jats:named-content>\n species in 27.1% of all samples tested.\n Cryptosporidium parvum<\/jats:named-content>\n - and\n C. hominis<\/jats:named-content>\n -positive isolates were genotyped using two nested-PCR assays that amplify the\n Cryptosporidium<\/jats:named-content>\n glycoprotein 60 gene (\n GP60<\/jats:italic>\n ).\n GP60<\/jats:italic>\n gene analysis showed the presence of two\n Cryptosporidium<\/jats:named-content>\n genotypes, namely,\n C. parvum<\/jats:named-content>\n IIaA19G1R1 and\n C. hominis<\/jats:named-content>\n IbA10G2. This study reveals a higher diversity of\n Cryptosporidium<\/jats:named-content>\n species\/genotypes than was previously expected. We suggest reasons for the high diversity of\n Cryptosporidium<\/jats:named-content>\n parasites within this isolated population and discuss the implications for our understanding of cryptosporidiosis.\n <\/jats:p>","DOI":"10.1128\/aem.02823-12","type":"journal-article","created":{"date-parts":[[2013,1,26]],"date-time":"2013-01-26T02:32:43Z","timestamp":1359167563000},"page":"2240-2246","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Genetic Diversity of Cryptosporidium spp. within a Remote Population of Soay Sheep on St. Kilda Islands, Scotland"],"prefix":"10.1128","volume":"79","author":[{"given":"L.","family":"Connelly","sequence":"first","affiliation":[{"name":"Scottish Parasite Diagnostic and Reference Laboratory, Stobhill Hospital, Glasgow, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. 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