{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:49:37Z","timestamp":1762271377812},"reference-count":39,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2009,10,15]],"date-time":"2009-10-15T00:00:00Z","timestamp":1255564800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2009,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We developed a single-plasmid-based regulatable protein expression system for\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            . Expression of a target gene is driven by P\n            <jats:sub>\n              <jats:italic>ost<\/jats:italic>\n            <\/jats:sub>\n            , a hybrid\n            <jats:italic>B. burgdorferi ospA<\/jats:italic>\n            -\n            <jats:italic>tetO<\/jats:italic>\n            promoter, from a recombinant\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            plasmid constitutively expressing TetR. The system was tested using the green fluorescent protein (GFP) as a reporter. Under noninducing conditions, recombinant\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            cells were nonfluorescent, no GFP protein was detected, and residual, small amounts of transcript were detectable only by reverse transcription-PCR but not by Northern blot hybridization. Upon induction with anhydrotetracycline, increasing levels of GFP transcript, protein, and fluorescence were observed. This tight and titratable promoter system will be invaluable for the study of essential borrelial proteins. Since target protein, operator, and repressor are carried by a single plasmid, the system's application is independent of a particular strain background.\n          <\/jats:p>","DOI":"10.1128\/aem.02825-08","type":"journal-article","created":{"date-parts":[[2009,8,22]],"date-time":"2009-08-22T01:50:29Z","timestamp":1250905829000},"page":"6553-6558","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Development of a Single-Plasmid-Based Regulatable Gene Expression System for\n            <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"75","author":[{"given":"Christine R.","family":"Whetstine","sequence":"first","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joyce G.","family":"Slusser","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfram R.","family":"Zu\u0308ckert","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.9.2745-2756.2004"},{"key":"e_1_3_2_3_2","first-page":"521","volume":"57","year":"1984","unstructured":"Barbour, A. 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