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However, other than in<jats:italic>Neisseria gonorrhoeae<\/jats:italic>, the transcriptional regulator FarR is not involved in regulation of this operon in<jats:italic>Neisseria meningitidis<\/jats:italic>. We tested the susceptibility of 23 meningococcal isolates against saturated and unsaturated long-chain fatty acids, proving that meningococci are generally highly resistant, with the exception of serogroup Y strains belonging to sequence type 23. Using genetically determined lipopolysaccharide (LPS)-truncated mutant strains, we show that addition of the LPS core oligosaccharide and hexa-acylation of its membrane anchor lipid A are imperative for fatty acid resistance of meningococci. The sensitivity of the serogroup Y strains is due to naturally occurring mutations within the<jats:italic>lpxL1<\/jats:italic>gene, which is responsible for addition of the sixth acyl chain on the LPS membrane anchor lipid A. Therefore, fatty acid resistance in meningococci is provided by both the active efflux pump FarAB and by the natural permeability barrier of the Gram-negative outer membrane. The transcriptional regulator FarR is not implicated in fatty acid resistance in meningococci, possibly giving rise to a constitutively active FarAB efflux pump system and thus revealing diverse mechanisms of niche adaptation in the two closely related<jats:italic>Neisseria<\/jats:italic>species.<\/jats:p>","DOI":"10.1128\/aem.02833-09","type":"journal-article","created":{"date-parts":[[2010,3,27]],"date-time":"2010-03-27T02:54:25Z","timestamp":1269658465000},"page":"3160-3169","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["The Transcriptional Repressor FarR Is Not Involved in Meningococcal Fatty Acid Resistance Mediated by the FarAB Efflux Pump and Dependent on Lipopolysaccharide Structure"],"prefix":"10.1128","volume":"76","author":[{"given":"Stephanie","family":"Schielke","sequence":"first","affiliation":[{"name":"University of Wu\u0308rzburg, Institute of Hygiene and Microbiology, Wu\u0308rzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinna","family":"Schmitt","sequence":"additional","affiliation":[{"name":"University of Wu\u0308rzburg, Institute of Hygiene and Microbiology, Wu\u0308rzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolin","family":"Spatz","sequence":"additional","affiliation":[{"name":"University of Wu\u0308rzburg, Institute of Hygiene and Microbiology, Wu\u0308rzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Frosch","sequence":"additional","affiliation":[{"name":"University of Wu\u0308rzburg, Institute of Hygiene and Microbiology, Wu\u0308rzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Schubert-Unkmeir","sequence":"additional","affiliation":[{"name":"University of Wu\u0308rzburg, Institute of Hygiene and Microbiology, Wu\u0308rzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Kurzai","sequence":"additional","affiliation":[{"name":"University of Wu\u0308rzburg, Institute of Hygiene and Microbiology, Wu\u0308rzburg, Germany"},{"name":"Friedrich Schiller University Jena, Septomics Research Centre, Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.3.646"},{"key":"e_1_3_2_3_2","first-page":"9677","volume":"274","year":"1999","unstructured":"Carty, S. 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