{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T10:49:51Z","timestamp":1763808591684},"reference-count":43,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Haemophilus\nparasuis<\/jats:italic>\n            is the cause of Gl\u00e4sser's disease and other\nclinical disorders in pigs. It can also be isolated from the upper\nrespiratory tracts of healthy pigs, and isolates can have significant\ndifferences in virulence. In this work, a partial sequence from the\n60-kDa heat shock protein (Hsp60) gene was assessed as an\nepidemiological marker. We analyzed partial sequences of\n            <jats:italic>hsp60<\/jats:italic>\n            and 16S rRNA genes from 103 strains of\n            <jats:italic>H. parasuis<\/jats:italic>\n            and other\nrelated species to obtain a better classification of the strains and\nexamine the correlation with virulence. The results were compared with\nthose obtained by enterobacterial repetitive intergenic consensus PCR.\nOur results showed that\n            <jats:italic>hsp60<\/jats:italic>\n            is a reliable marker for\nepidemiological studies of\n            <jats:italic>H. parasuis<\/jats:italic>\n            and that the analysis\nof its sequence is a better approach than fingerprinting methods.\nFurthermore, the analysis of the\n            <jats:italic>hsp60<\/jats:italic>\n            and 16S rRNA gene\nsequences revealed the presence of a separate lineage of virulent\nstrains and indicated the occurrence of lateral gene transfer among\n            <jats:italic>H. parasuis<\/jats:italic>\n            and\n            <jats:italic>Actinobacillus<\/jats:italic>\n            strains.\n          <\/jats:p>","DOI":"10.1128\/aem.02834-05","type":"journal-article","created":{"date-parts":[[2006,6,2]],"date-time":"2006-06-02T20:36:26Z","timestamp":1149280586000},"page":"3984-3992","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Genotypic Diversity of\n            <i>Haemophilus parasuis<\/i>\n            Field Strains"],"prefix":"10.1128","volume":"72","author":[{"given":"A.","family":"Olvera","sequence":"first","affiliation":[{"name":"Centre\rde Recerca en Sanitat Animal (CReSA), Campus de Bellaterra-Universitat\rAut\u00f2noma de Barcelona, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Calsamiglia","sequence":"additional","affiliation":[{"name":"Centre\rde Recerca en Sanitat Animal (CReSA), Campus de Bellaterra-Universitat\rAut\u00f2noma de Barcelona, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Aragon","sequence":"additional","affiliation":[{"name":"Centre\rde Recerca en Sanitat Animal (CReSA), Campus de Bellaterra-Universitat\rAut\u00f2noma de Barcelona, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.58.559"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.56.639"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306366101"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(03)00112-4"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.10.015"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(96)01342-9"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.06.011"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.10.4828-4833.2001"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.2001.00433.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00139"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00362-0"},{"key":"e_1_3_2_14_2","first-page":"55","volume":"80","year":"2005","unstructured":"Del Rio, M. L., C. B. Martin, J. Navas, B. Gutierrez-Muniz, J. I. Rodriguez-Barbosa, and E. F. Rodriguez Ferri. 2005. aroA gene PCR-RFLP diversity patterns in Haemophilus parasuis and Actinobacillus species. Res. Vet. Sci.80:55-61.","journal-title":"Res. Vet. Sci."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.6.2002-2013.1992"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a004174"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1420-9101.2004.00861.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-42-1-166"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.4.818-823.1996"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.12.3116-3121.1997"},{"key":"e_1_3_2_21_2","unstructured":"Graur D. and W. H. Li. 1999. Fundamentals of\nmolecular evolution 2nd ed. Sinauer Associates Sunderland Mass."},{"key":"e_1_3_2_22_2","first-page":"1621","volume":"30","year":"1969","unstructured":"Harris, D. L., R. F. Ross, and W. P. Switzer. 1969 . Incidence of certain microorganisms in nasal cavities of swine in Iowa. Am. J. Vet. Res.30:1621-1624.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_23_2","first-page":"58","volume":"7","year":"1999","unstructured":"Hoffman, P. S., and R. A. Garduno. 1999. Surface-associated heat shock proteins of Legionella pneumophila and Helicobacter pylori: roles in pathogenesis and immunity. Infect. Dis. Obstet. Gynecol.7:58-63.","journal-title":"Infect. Dis. Obstet. Gynecol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.6.1887-1891.2002"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.4.862-865.1992"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(01)00351-0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.55.1.709"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.12.1244"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.5.1927-1931.2005"},{"key":"e_1_3_2_30_2","first-page":"37","volume":"55","year":"1991","unstructured":"Miniats, O. P., N. L. Smart, and S. Rosendal. 1991 . Cross protection among Haemophilus parasuis strains in immunized gnotobiotic pigs. Can. J. Vet. Res.55:37-41.","journal-title":"Can. J. Vet. Res."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-4-951"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.12.2711-2716.1990"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.23.1.138-142.1986"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-36-1-17"},{"key":"e_1_3_2_35_2","first-page":"426","year":"1986","unstructured":"Nicolet, J. 1986. Haemophilus infection, p.426 -436. In A. D. Leman et al. (ed.), Diseases of swine, 6th ed. Iowa State University Press, Ames, Iowa.","journal-title":"Diseases of swine"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2003.64.435"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2003.12.001"},{"key":"e_1_3_2_38_2","unstructured":"Ooyen A. V. 2001. New approaches for the\ngeneration and analysis of microbial fingerprints. Elsevier Amsterdam The\nNetherlands."},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.2000.tb10507.x"},{"key":"e_1_3_2_40_2","first-page":"475","year":"1999","unstructured":"Rapp-Gabrielson, V. J. 1999. Haemophilus parasuis, p. 475-481. In B. E. Straw, S. D'Allaire, W. L. Mengeling, and D. J. Taylor (ed.), Diseases of swine. Iowa State University Press, Ames, Iowa.","journal-title":"Diseases of swine"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-44-4-846"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-37-4-463"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb10994.x"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02834-05","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02834-05","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T02:16:55Z","timestamp":1645582615000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.02834-05"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6]]},"references-count":43,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,6]]}},"alternative-id":["10.1128\/AEM.02834-05"],"URL":"https:\/\/doi.org\/10.1128\/aem.02834-05","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,6]]},"assertion":[{"value":"2005-12-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-03-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-06-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}