{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:18:47Z","timestamp":1791249527574,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2010,7,15]],"date-time":"2010-07-15T00:00:00Z","timestamp":1279152000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We describe a modification of the most probable number (MPN) method for rapid enumeration of antimicrobial-resistant\n            <jats:italic>Escherichia<\/jats:italic>\n            <jats:italic>coli<\/jats:italic>\n            bacteria in aqueous environmental samples.\n            <jats:italic>E. coli<\/jats:italic>\n            (total and antimicrobial-resistant) bacteria were enumerated in effluent samples from a hospital (\n            <jats:italic>n<\/jats:italic>\n            = 17) and municipal sewers upstream (\n            <jats:italic>n<\/jats:italic>\n            = 5) and downstream (\n            <jats:italic>n<\/jats:italic>\n            = 5) from the hospital, effluent samples from throughout the treatment process (\n            <jats:italic>n<\/jats:italic>\n            = 4), and treated effluent samples (\n            <jats:italic>n<\/jats:italic>\n            = 13). Effluent downstream from the hospital contained a higher proportion of antimicrobial-resistant\n            <jats:italic>E. coli<\/jats:italic>\n            than that upstream from the hospital. Wastewater treatment reduced the numbers of\n            <jats:italic>E. coli<\/jats:italic>\n            bacteria (total and antimicrobial resistant); however, antimicrobial-resistant\n            <jats:italic>E. coli<\/jats:italic>\n            was not eliminated, and\n            <jats:italic>E. coli<\/jats:italic>\n            resistant to cefotaxime (including extended-spectrum beta-lactamase [ESBL] producers), ciprofloxacin, and cefoxitin was present in treated effluent samples.\n          <\/jats:p>","DOI":"10.1128\/aem.02898-09","type":"journal-article","created":{"date-parts":[[2010,6,5]],"date-time":"2010-06-05T02:22:43Z","timestamp":1275704563000},"page":"4772-4779","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":208,"title":["Enumeration and Characterization of Antimicrobial-Resistant\n            <i>Escherichia coli<\/i>\n            Bacteria in Effluent from Municipal, Hospital, and Secondary Treatment Facility Sources"],"prefix":"10.1128","volume":"76","author":[{"given":"Sandra","family":"Galvin","sequence":"first","affiliation":[{"name":"School of Medicine, National University of Ireland, Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Boyle","sequence":"additional","affiliation":[{"name":"School of Medicine, National University of Ireland, Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Hickey","sequence":"additional","affiliation":[{"name":"Environmental Health Office, Health Services Executive Western Region, Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akke","family":"Vellinga","sequence":"additional","affiliation":[{"name":"School of Medicine, National University of Ireland, Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dearbha\u0301ile","family":"Morris","sequence":"additional","affiliation":[{"name":"School of Medicine, National University of Ireland, Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Cormican","sequence":"additional","affiliation":[{"name":"School of Medicine, National University of Ireland, Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.42.2.277-283.1981"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2003.09.038"},{"key":"e_1_3_2_4_2","unstructured":"Performance standards for antimicrobial disk susceptibility test; 15th informational supplement. 2005"},{"key":"e_1_3_2_5_2","first-page":"2386","year":"2005","unstructured":"Donnenberg, M. 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