{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:02:06Z","timestamp":1790881326768,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2016,3,15]],"date-time":"2016-03-15T00:00:00Z","timestamp":1458000000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["H21-Shinkou-Ippan-008"],"award-info":[{"award-number":["H21-Shinkou-Ippan-008"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["H24-Shinkou-Ippan-010"],"award-info":[{"award-number":["H24-Shinkou-Ippan-010"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The actual state of intestinal long-term colonization by extended-spectrum \u03b2-lactamase (ESBL)-producing\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            in healthy Japanese people remains unclear. Therefore, a total of 4,314 fecal samples were collected from 2,563 food handlers from January 2010 to December 2011. Approximately 0.1 g of each fecal sample was inoculated onto a MacConkey agar plate containing cefotaxime (1 \u03bcg\/ml). The bacterial colonies that grew on each plate were checked for ESBL production by the double-disk synergy test, as recommended by the Clinical and Laboratory Standards Institute. The bacterial serotype, antimicrobial susceptibility, pulsotype, sequence type (ST), and ESBL genotype were checked, and the replicon types of plasmids harboring the ESBL gene were also determined after conjugation experiments. ESBL producers were recovered from 70 (3.1%) of 2,230 participants who were checked only once. On the other hand, ESBL producers were isolated at least once from 52 (15.6%) of 333 participants who were checked more than twice, and 13 of the 52 participants carried ESBL producers for from more than 3 months to up to 2 years. Fluoroquinolone (FQ)-resistant\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            strains harboring\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            were repeatedly recovered from 11 of the 13 carriers of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            -harboring\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            . A genetically related FQ-resistant\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            O25b:H4-ST131 isolate harboring\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-27<\/jats:sub>\n            was recovered from 4 of the 13 carriers for more than 6 months. Three FQ-resistant\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            O1:H6-ST648 isolates that harbored\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            or\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-14<\/jats:sub>\n            were recovered from 3 carriers. Moreover, multiple CTX-M-14- or CTX-M-15-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            isolates with different serotypes were recovered from 2 respective carriers. These findings predict a provable further spread of ESBL producers in both community and clinical settings.\n          <\/jats:p>","DOI":"10.1128\/aem.02929-15","type":"journal-article","created":{"date-parts":[[2016,1,9]],"date-time":"2016-01-09T03:59:34Z","timestamp":1452311974000},"page":"1818-1827","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Long-Term Colonization by\n            <i>bla<\/i>\n            <sub>CTX-M<\/sub>\n            -Harboring Escherichia coli in Healthy Japanese People Engaged in Food Handling"],"prefix":"10.1128","volume":"82","author":[{"given":"Kunihiko","family":"Nakane","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan"},{"name":"Okazaki City Public Health Center, Okazaki, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumiko","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Department of Pathophysiological Laboratory Science, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kensuke","family":"Goto","sequence":"additional","affiliation":[{"name":"Department of Pathophysiological Laboratory Science, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshichika","family":"Arakawa","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.4.657-686.2005"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01850.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70041-0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.059279-0"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00023-13"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-014-2141-8"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2458-14-410"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.129810"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12592"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.2900"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/533475"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2011.592987"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2011.653582"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12559"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn463"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp498"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb08989.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn084"},{"key":"e_1_3_3_20_2","volume-title":"Approved standard M7-A9","author":"Clinical and Laboratory Standards Institute","year":"2011","unstructured":"Clinical and Laboratory Standards Institute. 2011. 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