{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:20:47Z","timestamp":1758709247679,"version":"3.41.2"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2017,3,15]],"date-time":"2017-03-15T00:00:00Z","timestamp":1489536000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1510131"],"award-info":[{"award-number":["1510131"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Science Foundation (NSF) Graduate Research Fellowship"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2017,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Organohalide-respiring bacteria can be difficult to enrich and isolate, which can limit research on these important organisms. The goal of this research was to develop a method to rapidly (minutes to days) enrich these organisms from a mixed community. The method presented is based on the hypothesis that organohalide-respiring bacteria would be more hydrophobic than other bacteria as they dehalogenate hydrophobic compounds. The method developed tests this hypothesis by separating a portion of putative organohalide-respiring bacteria, those phylogenetically related to\n            <jats:named-content content-type=\"genus-species\">Dehalococcoides mccartyi<\/jats:named-content>\n            , at the interface between a hydrophobic organic solvent and an aqueous medium. This novel partial separation technique was tested with a polychlorinated biphenyl-enriched sediment-free culture, a tetrachloroethene-enriched digester sludge culture, and uncontaminated lake sediment. Significantly higher fractions, up to 20.4 times higher, of putative organohalide-respiring bacteria were enriched at the interface between the medium and either hexadecane or trichloroethene. The selective partial separation of these putative organohalide-respiring bacteria occurred after 20 min, strongly suggesting that the separation was a result of physical-chemical interactions between the cell surface and hydrophobic solvent. Dechlorination activity postseparation was verified by the production of\n            <jats:italic>cis<\/jats:italic>\n            -dichloroethene when amended with tetrachloroethene. A longer incubation time of 6 days prior to separation with trichloroethene increased the total number of putative organohalide-respiring bacteria. This method provides a way to quickly separate some of the putative organohalide-respiring bacteria from other bacteria, thereby improving our ability to study multiple and different bacteria of potential interest and improving knowledge of these bacteria.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Organohalide-respiring bacteria, bacteria capable of respiring chlorinated contaminants, can be difficult to enrich, which can limit their predictable use for the bioremediation of contaminated sites. This paper describes a method to quickly separate\n            <jats:named-content content-type=\"genus-species\">Dehalococcoides<\/jats:named-content>\n            -like bacteria, a group of organisms containing organohalide-respiring bacteria, from other bacteria in a mixed community. From this work,\n            <jats:named-content content-type=\"genus-species\">Dehalococcoides<\/jats:named-content>\n            -like bacteria appear to have a hydrophobic cell surface, facilitating a rapid (20 min) partial separation from a mixed culture at the surface of a hydrophobic liquid. This method was verified in a polychlorinated biphenyl-enriched sediment-free culture, an anaerobic digester sludge, and uncontaminated sediment. The method described can drastically reduce the amount of time required to partially separate\n            <jats:named-content content-type=\"genus-species\">Dehalococcoides<\/jats:named-content>\n            -like bacteria from a complex mixed culture, improving researchers' ability to study these important bacteria.\n          <\/jats:p>","DOI":"10.1128\/aem.02946-16","type":"journal-article","created":{"date-parts":[[2017,1,14]],"date-time":"2017-01-14T02:40:30Z","timestamp":1484361630000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Rapid Enrichment of Dehalococcoides-Like Bacteria by Partial Hydrophobic Separation"],"prefix":"10.1128","volume":"83","author":[{"given":"Hanna R.","family":"Temme","sequence":"first","affiliation":[{"name":"Department of Civil, Environmental, and Geo-Engineering, University of Minnesota, Minneapolis, Minnesota, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kipp","family":"Sande","sequence":"additional","affiliation":[{"name":"Department of Civil, Environmental, and Geo-Engineering, University of Minnesota, Minneapolis, Minnesota, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Hawaii at Manoa, Honolulu, Hawaii, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paige J.","family":"Novak","sequence":"additional","affiliation":[{"name":"Department of Civil, Environmental, and Geo-Engineering, University of Minnesota, Minneapolis, Minnesota, 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