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SalR is a typical LysR-type transcriptional regulator (LTTR) family protein and activates the\n            <jats:italic>\n              P\n              <jats:sub>sal<\/jats:sub>\n            <\/jats:italic>\n            promoter in the presence of aspirin or salicylate in the range of 0.05 to 10\u2009\u00b5M. The experimental results and mathematical simulations support the competitive binding model of the SalR\/\n            <jats:italic>\n              P\n              <jats:sub>sal<\/jats:sub>\n            <\/jats:italic>\n            regulation system in which SalR\n            <jats:sub>r<\/jats:sub>\n            competes with SalR\n            <jats:sub>a<\/jats:sub>\n            to bind the\n            <jats:italic>\n              P\n              <jats:sub>sal<\/jats:sub>\n            <\/jats:italic>\n            promoter and affect gene transcription. The competitive binding model successfully predicted that weak SalR expression would significantly improve the inducible strength of the SalR\/\n            <jats:italic>\n              P\n              <jats:sub>sal<\/jats:sub>\n            <\/jats:italic>\n            regulation system, which is confirmed by the experimental results. This provides an important mechanism model to fine-tune transcriptional regulation of the LTTR family, which is the largest family of transcriptional regulators in the prokaryotic kingdom. In addition, the SalR\/\n            <jats:italic>\n              P\n              <jats:sub>sal<\/jats:sub>\n            <\/jats:italic>\n            regulation system was also functional in probiotic strain\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            Nissle 1917 and minicell-derived SimCells, which would be a useful biobrick for environmental and medical applications.\n          <\/jats:p>","DOI":"10.1128\/aem.02959-18","type":"journal-article","created":{"date-parts":[[2019,1,16]],"date-time":"2019-01-16T14:05:59Z","timestamp":1547647559000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Development of Aspirin-Inducible Biosensors in\n            <i>Escherichia coli<\/i>\n            and SimCells"],"prefix":"10.1128","volume":"85","author":[{"given":"Jack Xiaoyu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Engineering Science, University of Oxford, Oxford, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harrison","family":"Steel","sequence":"additional","affiliation":[{"name":"Department of Engineering Science, University of Oxford, Oxford, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin-Hu","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Engineering Science, University of Oxford, Oxford, United Kingdom"},{"name":"Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Engineering Science, University of Oxford, Oxford, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiabao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Engineering Science, University of Oxford, Oxford, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cordelia P. 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