{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:13:14Z","timestamp":1790673194832,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Streptococcus suis<\/jats:named-content>\n                    is an emerging zoonotic pathogen causing severe infections in pigs and humans. In previous studies, 33 serotypes of\n                    <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n                    have been identified using serum agglutination. Here, we describe a novel\n                    <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n                    strain, CZ130302, isolated from an outbreak of acute piglet meningitis in eastern China. Strong pathogenicity of meningitis caused by strain CZ130302 was reproduced in the BALB\/c mouse model. The strain showed a high fatality rate (8\/10), higher than those for known virulent serotype 2 strains P1\/7 (1\/10) and 9801 (2\/10). Cell adhesion assay results with bEnd.3 and HEp2 cells showed that CZ130302 was significantly close to P1\/7 and 9801. Both the agglutination test and its complementary test showed that strain CZ130302 had no strong cross-reaction with the other 33\n                    <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n                    serotypes. The multiplex PCR assays revealed no specified bands for all four sets used to detect the other 33 serotypes. In addition, genetic analysis of the whole\n                    <jats:italic>cps<\/jats:italic>\n                    gene clusters of all serotypes was performed in this study. The results of comparative genomics showed that the\n                    <jats:italic>cps<\/jats:italic>\n                    gene cluster of CZ130302, which was not previously reported, showed no homology to the gene sequences of the other strains. Especially, the\n                    <jats:italic>wzy<\/jats:italic>\n                    ,\n                    <jats:italic>wzx<\/jats:italic>\n                    , and acetyltransferase genes of strain CZ130302 are phylogenetically distinct from strains of the other 33 serotypes. Therefore, this study suggested that strain CZ130302 represents a novel variant serotype of\n                    <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n                    (designated serotype Chz) which has a high potential to be virulent and associated with meningitis in animals.\n                  <\/jats:p>","DOI":"10.1128\/aem.02962-14","type":"journal-article","created":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T23:40:10Z","timestamp":1416613210000},"page":"976-985","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":83,"title":["Novel Variant Serotype of Streptococcus suis Isolated from Piglets with Meningitis"],"prefix":"10.1128","volume":"81","author":[{"given":"Zihao","family":"Pan","sequence":"first","affiliation":[{"name":"Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China"},{"name":"OIE Reference Lab for Swine Streptococcosis, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiale","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China"},{"name":"OIE Reference Lab for Swine Streptococcosis, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyang","family":"Dong","sequence":"additional","affiliation":[{"name":"OIE Reference Lab for Swine Streptococcosis, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenchao","family":"Song","sequence":"additional","affiliation":[{"name":"OIE Reference Lab for Swine Streptococcosis, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaicheng","family":"Wang","sequence":"additional","affiliation":[{"name":"China Animal Health and Epidemiology Center, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengping","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China"},{"name":"OIE Reference Lab for Swine Streptococcosis, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huochun","family":"Yao","sequence":"additional","affiliation":[{"name":"Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China"},{"name":"OIE Reference Lab for Swine Streptococcosis, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.11.149"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1968.tb03456.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1909.121872"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.046599-0"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3671-3680.2002"},{"key":"e_1_3_3_7_2","first-page":"868","article-title":"Streptococcus suis meningitis in Thailand","volume":"35","author":"Suankratay C","year":"2004","unstructured":"Suankratay C, Intalapaporn P, Nunthapisud P, Arunyingmongkol K, Wilde H. 2004. 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Is Kaplan-Meier statistics the most appropriate tool for survivorship measurement of outcomes in orthopaedics? Acta Chir Orthop Traumatol Cech 77:118\u2013123. 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