{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:24:38Z","timestamp":1791185078968,"version":"4.1.0"},"reference-count":71,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2011,6,15]],"date-time":"2011-06-15T00:00:00Z","timestamp":1308096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We propose the use of\n                    <jats:named-content content-type=\"genus-species\">Desulfovibrio desulfuricans<\/jats:named-content>\n                    ND132 as a model species for understanding the mechanism of microbial Hg methylation. Strain ND132 is an anaerobic dissimilatory sulfate-reducing bacterium (DSRB), isolated from estuarine mid-Chesapeake Bay sediments. It was chosen for study because of its exceptionally high rates of Hg methylation in culture and its metabolic similarity to the lost strain\n                    <jats:named-content content-type=\"genus-species\">D. desulfuricans<\/jats:named-content>\n                    LS, the only organism for which methylation pathways have been partially defined. Strain ND132 is an incomplete oxidizer of short-chain fatty acids. It is capable of respiratory growth using fumarate as an electron acceptor, supporting growth without sulfide production. We used enriched stable Hg isotopes to show that ND132 simultaneously produces and degrades methylmercury (MeHg) during growth but does not produce elemental Hg. MeHg produced by cells is mainly excreted, and no MeHg is produced in spent medium. Mass balances for Hg and MeHg during the growth of cultures, including the distribution between filterable and particulate phases, illustrate how medium chemistry and growth phase dramatically affect Hg solubility and availability for methylation. The available information on Hg methylation among strains in the genus\n                    <jats:named-content content-type=\"genus-species\">Desulfovibrio<\/jats:named-content>\n                    is summarized, and we present methylation rates for several previously untested species. About 50% of\n                    <jats:named-content content-type=\"genus-species\">Desulfovibrio<\/jats:named-content>\n                    strains tested to date have the ability to produce MeHg. Importantly, the ability to produce MeHg is constitutive and does not confer Hg resistance. A 16S rRNA-based alignment of the genus\n                    <jats:named-content content-type=\"genus-species\">Desulfovibrio<\/jats:named-content>\n                    allows the very preliminary assessment that there may be some evolutionary basis for the ability to produce MeHg within this genus.\n                  <\/jats:p>","DOI":"10.1128\/aem.02993-10","type":"journal-article","created":{"date-parts":[[2011,4,22]],"date-time":"2011-04-22T23:29:25Z","timestamp":1303514965000},"page":"3938-3951","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":292,"title":["Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury Methylation"],"prefix":"10.1128","volume":"77","author":[{"given":"Cynthia C.","family":"Gilmour","sequence":"first","affiliation":[{"name":"Smithsonian Environmental Research Center, 647 Contees Wharf Rd., Edgewater, Maryland 21037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dwayne A.","family":"Elias","sequence":"additional","affiliation":[{"name":"BioSciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy M.","family":"Kucken","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Microbiology &amp; Immunology, University of Missouri\u2014Columbia, Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven D.","family":"Brown","sequence":"additional","affiliation":[{"name":"BioSciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony V.","family":"Palumbo","sequence":"additional","affiliation":[{"name":"BioSciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher W.","family":"Schadt","sequence":"additional","affiliation":[{"name":"BioSciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy D.","family":"Wall","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Microbiology &amp; Immunology, University of Missouri\u2014Columbia, Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.9303"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00406665"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.11.4267-4271.1997"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/om9508391"},{"key":"e_1_3_3_6_2","first-page":"262","volume-title":"Biogeochemistry of environmentally important trace elements. 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