{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T15:01:22Z","timestamp":1778857282167,"version":"3.51.4"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Some\n            <jats:named-content content-type=\"genus-species\">Arcobacter<\/jats:named-content>\n            species are considered emerging food-borne and waterborne pathogens, and shellfish have been suggested as one of their reservoirs. However, only a few studies have investigated the presence of\n            <jats:named-content content-type=\"genus-species\">Arcobacter<\/jats:named-content>\n            in this kind of food. This study assesses the prevalence and diversity of\n            <jats:named-content content-type=\"genus-species\">Arcobacter<\/jats:named-content>\n            spp. in shellfish by multiplex PCR (m-PCR) and culturing methods (under different atmospheric conditions) and evaluates the possible influence of environmental parameters (temperature, salinity, and harvesting bay).\n            <jats:named-content content-type=\"genus-species\">Arcobacter<\/jats:named-content>\n            was detected by m-PCR and\/or culturing in 61 (29.9%) of 204 shellfish samples. Of the positive samples by culturing, 41.1% were obtained under only aerobic incubation conditions, while 23.2% were obtained under only microaerobic conditions. Of 476 investigated isolates, 118 belonged to different enterobacterial repetitive intergenic consensus (ERIC)-PCR genotypes (strains) and to 11 different species. This study shows the highest diversity of\n            <jats:named-content content-type=\"genus-species\">Arcobacter<\/jats:named-content>\n            species ever observed in samples from any origin. The most prevalent species was\n            <jats:named-content content-type=\"genus-species\">Arcobacter butzleri<\/jats:named-content>\n            (60.2%), followed by\n            <jats:named-content content-type=\"genus-species\">Arcobacter molluscorum<\/jats:named-content>\n            (21.2%). The prevalence of\n            <jats:named-content content-type=\"genus-species\">Arcobacter<\/jats:named-content>\n            was significantly higher during the summer than in other seasons, being associated with an increase in water temperature. Results confirm that shellfish are a reservoir for a remarkable diversity of\n            <jats:named-content content-type=\"genus-species\">Arcobacter<\/jats:named-content>\n            spp.\n          <\/jats:p>","DOI":"10.1128\/aem.03014-13","type":"journal-article","created":{"date-parts":[[2013,11,2]],"date-time":"2013-11-02T00:48:09Z","timestamp":1383353289000},"page":"385-391","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Higher Water Temperature and Incubation under Aerobic and Microaerobic Conditions Increase the Recovery and Diversity of Arcobacter spp. from Shellfish"],"prefix":"10.1128","volume":"80","author":[{"given":"Arturo","family":"Levican","sequence":"first","affiliation":[{"name":"Unitat de Microbiologia, Departament de Ci\u00e8ncies Mediques B\u00e0siques, Facultat de Medicina i Ci\u00e8ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Collado","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, Departament de Ci\u00e8ncies Mediques B\u00e0siques, Facultat de Medicina i Ci\u00e8ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"},{"name":"Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Yustes","sequence":"additional","affiliation":[{"name":"Ag\u00e8ncia de Salut P\u00fablica de Catalunya (ASPCAT), Laboratori de l'ASPCAT, Tarragona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carme","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Ag\u00e8ncia de Salut P\u00fablica de Catalunya (ASPCAT), Laboratori de l'ASPCAT, Tarragona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Jos\u00e9","family":"Figueras","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, Departament de Ci\u00e8ncies Mediques B\u00e0siques, Facultat de Medicina i Ci\u00e8ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-41-1-88"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.03.004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00034-10"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.054155-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-72.5.1102"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.7.2691-2696.1998"},{"key":"e_1_3_2_8_2","first-page":"143","article-title":"Isolation of Arcobacter spp. from a brackish environment","volume":"23","author":"Maugeri TL","year":"2000","unstructured":"MaugeriTLGugliandoloCCarboneMCaccamoDFeraMT. 2000. 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