{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:40:28Z","timestamp":1782978028852,"version":"3.54.5"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2010,7,15]],"date-time":"2010-07-15T00:00:00Z","timestamp":1279152000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The cometabolic degradation of trichloroethene (TCE) by\n            <jats:italic>Rhodococcus<\/jats:italic>\n            sp. L4 was limited by the loss of enzyme activity during TCE transformation. This problem was overcome by repeated addition of inducing substrates, such as cumene, limonene, or cumin aldehyde, to the cells. Alternatively,\n            <jats:italic>Rhodococcus<\/jats:italic>\n            sp. L4 was immobilized on plant materials which contain those inducers in their essential oils. Cumin seeds were the most suitable immobilizing material, and the immobilized cells tolerated up to 68 \u03bcM TCE and degraded TCE continuously. The activity of immobilized cells, which had been inactivated partially during TCE degradation, could be reactivated by incubation in mineral salts medium without TCE. These findings demonstrate that immobilization of\n            <jats:italic>Rhodococcus<\/jats:italic>\n            sp. L4 on plant materials rich in essential oils is a promising method for efficient cometabolic degradation of TCE.\n          <\/jats:p>","DOI":"10.1128\/aem.03036-09","type":"journal-article","created":{"date-parts":[[2010,5,15]],"date-time":"2010-05-15T02:01:39Z","timestamp":1273888899000},"page":"4684-4690","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Cometabolic Degradation of Trichloroethene by\n            <i>Rhodococcus<\/i>\n            sp. Strain L4 Immobilized on Plant Materials Rich in Essential Oils"],"prefix":"10.1128","volume":"76","author":[{"given":"Oramas","family":"Suttinun","sequence":"first","affiliation":[{"name":"International Postgraduate Programs in Environmental Management, Graduate School, Chulalongkorn University, Bangkok, Thailand"},{"name":"National Center of Excellence for Environmental and Hazardous Waste Management (NCE-EHWM), Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Mu\u0308ller","sequence":"additional","affiliation":[{"name":"Institute of Technical Biocatalysis, Technical University Hamburg-Harburg, Denickestrasse 15, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekawan","family":"Luepromchai","sequence":"additional","affiliation":[{"name":"National Center of Excellence for Environmental and Hazardous Waste Management (NCE-EHWM), Chulalongkorn University, Bangkok, Thailand"},{"name":"Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00020a003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012089908518"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01570068"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2007.03.030"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.3.853-860.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00249058"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00276540"},{"key":"e_1_3_2_9_2","first-page":"410","year":"1994","unstructured":"Focht, D. 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