{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:41:20Z","timestamp":1791175280431,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2010,10,15]],"date-time":"2010-10-15T00:00:00Z","timestamp":1287100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The use of natural compounds from plants can provide an alternative approach against food-borne pathogens. The mechanisms of action of most plant extracts with antimicrobial activity have been poorly studied. In this work, changes in membrane integrity, membrane potential, internal pH (pH\n            <jats:sub>in<\/jats:sub>\n            ), and ATP synthesis were measured in\n            <jats:italic>V<\/jats:italic>\n            <jats:italic>ibrio<\/jats:italic>\n            <jats:italic>cholerae<\/jats:italic>\n            cells after exposure to extracts of edible and medicinal plants. A preliminary screen of methanolic, ethanolic, and aqueous extracts of medicinal and edible plants was performed. Minimal bactericidal concentrations (MBCs) were measured for extracts showing high antimicrobial activity. Our results indicate that methanolic extracts of basil (\n            <jats:italic>Ocimum basilicum<\/jats:italic>\n            L.), nopal cactus (\n            <jats:italic>Opuntia ficus-indica<\/jats:italic>\n            var. Villanueva L.), sweet acacia (\n            <jats:italic>Acacia farnesiana<\/jats:italic>\n            L.), and white sagebrush (\n            <jats:italic>Artemisia ludoviciana<\/jats:italic>\n            Nutt.) are the most active against\n            <jats:italic>V. cholera<\/jats:italic>\n            , with MBCs ranging from 0.5 to 3.0 mg\/ml. Using four fluorogenic techniques, we studied the membrane integrity of\n            <jats:italic>V. cholerae<\/jats:italic>\n            cells after exposure to these four extracts. Extracts from these plants were able to disrupt the cell membranes of\n            <jats:italic>V. cholerae<\/jats:italic>\n            cells, causing increased membrane permeability, a clear decrease in cytoplasmic pH, cell membrane hyperpolarization, and a decrease in cellular ATP concentration in all strains tested. These four plant extracts could be studied as future alternatives to control\n            <jats:italic>V. cholerae<\/jats:italic>\n            contamination in foods and the diseases associated with this microorganism.\n          <\/jats:p>","DOI":"10.1128\/aem.03052-09","type":"journal-article","created":{"date-parts":[[2010,8,28]],"date-time":"2010-08-28T02:30:37Z","timestamp":1282962637000},"page":"6888-6894","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":224,"title":["Extracts of Edible and Medicinal Plants Damage Membranes of\n            <i>Vibrio cholerae<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Eduardo","family":"S\u00e1nchez","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Apdo. Postal 124-F, Ciudad Universitaria, San Nicol\u00e1s de los Garza N.L. 66451, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santos","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Apdo. Postal 124-F, Ciudad Universitaria, San Nicol\u00e1s de los Garza N.L. 66451, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma","family":"Heredia","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Apdo. Postal 124-F, Ciudad Universitaria, San Nicol\u00e1s de los Garza N.L. 66451, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"69","volume":"58","year":"2009","unstructured":"Adiguzel, A., H. Ozer, M. Sokmen, M. Gulluce, A. Sokmen, H. Kilic, F. Sahin, and O. Baris. 2009. Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria. Pol. J. Microbiol.58:69-76.","journal-title":"Pol. J. Microbiol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.147.3.820-826.1981"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02750-06"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02075"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-009-0507-0"},{"key":"e_1_3_2_7_2","first-page":"1563","volume":"8","year":"2004","unstructured":"Breeuwer, P., and T. Abee. 2004. 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