{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:54:18Z","timestamp":1791176058013,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2010,6,15]],"date-time":"2010-06-15T00:00:00Z","timestamp":1276560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>In vivo<\/jats:italic>\n            reconstitution of the TDP-\n            <jats:sc>l<\/jats:sc>\n            -megosamine pathway from the megalomicin gene cluster of\n            <jats:italic>Micromonospora megalomicea<\/jats:italic>\n            was accomplished by the heterologous expression of its biosynthetic genes in\n            <jats:italic>Escherichia coli.<\/jats:italic>\n            Mass spectrometric analysis of the TDP-sugar intermediates produced from operons containing different sets of genes showed that the production of TDP-\n            <jats:sc>l<\/jats:sc>\n            -megosamine from TDP-4-keto-6-deoxy-\n            <jats:sc>d<\/jats:sc>\n            -glucose requires only five biosynthetic steps, catalyzed by MegBVI, MegDII, MegDIII, MegDIV, and MegDV. Bioconversion studies demonstrated that the sugar transferase MegDI, along with the helper protein MegDVI, catalyzes the transfer of\n            <jats:sc>l<\/jats:sc>\n            -megosamine to either erythromycin C or erythromycin D, suggesting two possible routes for the production of megalomicin A. Analysis\n            <jats:italic>in vivo<\/jats:italic>\n            of the hydroxylation step by MegK indicated that erythromycin C is the intermediate of megalomicin A biosynthesis.\n          <\/jats:p>","DOI":"10.1128\/aem.03083-09","type":"journal-article","created":{"date-parts":[[2010,4,24]],"date-time":"2010-04-24T02:10:08Z","timestamp":1272075008000},"page":"3869-3877","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["TDP-\n            <scp>l<\/scp>\n            -Megosamine Biosynthesis Pathway Elucidation and Megalomicin A Production in\n            <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Mariana","family":"Useglio","sequence":"first","affiliation":[{"name":"Microbiology Division, IBR (Instituto de Biologi\u0301a Molecular y Celular de Rosario), Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvador","family":"Peiru\u0301","sequence":"additional","affiliation":[{"name":"Microbiology Division, IBR (Instituto de Biologi\u0301a Molecular y Celular de Rosario), Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Rodri\u0301guez","sequence":"additional","affiliation":[{"name":"Microbiology Division, IBR (Instituto de Biologi\u0301a Molecular y Celular de Rosario), Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo R.","family":"Labadie","sequence":"additional","affiliation":[{"name":"IQUIOS (Instituto de Qui\u0301mica Orga\u0301nica de Si\u0301ntesis, Departamento de Qui\u0301mica Orga\u0301nica), Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Carney","sequence":"additional","affiliation":[{"name":"Kosan Biosciences, Inc., 3832 Bay Center Place, Hayward, California 94545"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo","family":"Gramajo","sequence":"additional","affiliation":[{"name":"Microbiology Division, IBR (Instituto de Biologi\u0301a Molecular y Celular de Rosario), Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)80732-4"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2006.04.001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.10.2668"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.110.16.1839"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.7.3719"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja049967j"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja991713o"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.210395097"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi020245j"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja9837250"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2006.08.021"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2004.04.015"},{"key":"e_1_3_2_14_2","first-page":"1374","volume":"13","year":"1973","unstructured":"Jaret, R. 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