{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T03:33:52Z","timestamp":1791084832625,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We developed a high-throughput method based on terminal restriction fragment length polymorphisms (T-RFLP) to identify\n            <jats:italic>ospC<\/jats:italic>\n            genotypes from field-collected samples of\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            . We first validated the method by analyzing\n            <jats:italic>B. burgdorferi ospC<\/jats:italic>\n            previously identified by sequencing. We then analyzed and compared\n            <jats:italic>ospC<\/jats:italic>\n            genotypes detected from ear biopsy tissue from natural populations of the white-footed mouse, a major\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            reservoir host species in the eastern United States, and larval ticks feeding on those individual mice. The T-RFLP method enabled us to distinguish all 17\n            <jats:italic>ospC<\/jats:italic>\n            genotypes tested, as well as mixed samples containing more than one genotype. Analysis costs compare favorably to those of alternative\n            <jats:italic>ospC<\/jats:italic>\n            identification methods. The T-RFLP method will facilitate large-scale field studies to advance our understanding of genotype-specific transmission patterns.\n          <\/jats:p>","DOI":"10.1128\/aem.03106-12","type":"journal-article","created":{"date-parts":[[2012,11,27]],"date-time":"2012-11-27T05:57:27Z","timestamp":1353995847000},"page":"958-964","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Identification of\n            <i>Borrelia burgdorferi ospC<\/i>\n            Genotypes in Host Tissue and Feeding Ticks by Terminal Restriction Fragment Length Polymorphisms"],"prefix":"10.1128","volume":"79","author":[{"given":"Kimberly","family":"Tsao","sequence":"first","affiliation":[{"name":"Yale School of Public Health, New Haven, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Bent","sequence":"additional","affiliation":[{"name":"School of Molecular and Biomedical 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