{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T07:08:20Z","timestamp":1742800100792},"reference-count":61,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The development of novel intervention strategies for the control of zoonoses caused by bacteria such as\n            <jats:italic>Salmonella<\/jats:italic>\n            spp. in livestock requires appropriate experimental models to assess their suitability. Here, a novel porcine intestinal\n            <jats:italic>in vitro<\/jats:italic>\n            organ culture (IVOC) model utilizing cell crown (CC) technology (CCIVOC) (Scaffdex) was developed. The CCIVOC model was employed to investigate the characteristics of association of\n            <jats:italic>S. enterica<\/jats:italic>\n            serovar Typhimurium strain SL1344 with porcine intestinal tissue following exposure to a\n            <jats:italic>Lactobacillus plantarum<\/jats:italic>\n            strain. The association of bacteria to host cells was examined by light microscopy and electron microscopy (EM) after appropriate treatments and staining, while changes in the proteome of porcine jejunal tissues were investigated using quantitative label-free proteomics. Exposure of porcine intestinal mucosal tissues to\n            <jats:italic>L. plantarum<\/jats:italic>\n            JC1 did not reduce the numbers of\n            <jats:italic>S.<\/jats:italic>\n            Typhimurium bacteria associating to the tissues but was associated with significant (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.005) reductions in the percentages of areas of intestinal IVOC tissues giving positive staining results for acidic mucins. Conversely, the quantity of neutrally charged mucins present within the goblet cells of the IVOC tissues increased significantly (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05). In addition, tubulin-\u03b1 was expressed at high levels following inoculation of jejunal IVOC tissues with\n            <jats:italic>L. plantarum<\/jats:italic>\n            . Although\n            <jats:italic>L. plantarum<\/jats:italic>\n            JC1 did not reduce the association of\n            <jats:italic>S.<\/jats:italic>\n            Typhimurium strain SL1344 to the jejunal IVOC tissues, detection of increased acidic mucin secretion, host cytoskeletal rearrangements, and proteins involved in the porcine immune response demonstrated that this strain of\n            <jats:italic>L. plantarum<\/jats:italic>\n            may contribute to protecting the pig from infections by\n            <jats:italic>S.<\/jats:italic>\n            Typhimurium or other pathogens.\n          <\/jats:p>","DOI":"10.1128\/aem.03115-09","type":"journal-article","created":{"date-parts":[[2010,7,17]],"date-time":"2010-07-17T02:01:16Z","timestamp":1279332076000},"page":"6645-6657","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Response of Porcine Intestinal\n            <i>In Vitro<\/i>\n            Organ Culture Tissues following Exposure to\n            <i>Lactobacillus plantarum<\/i>\n            JC1 and\n            <i>Salmonella enterica<\/i>\n            Serovar Typhimurium SL1344"],"prefix":"10.1128","volume":"76","author":[{"given":"J. W.","family":"Collins","sequence":"first","affiliation":[{"name":"Department for Food and Environmental Safety, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom"},{"name":"The University of Reading, Department of Food and Nutritional Sciences, Whiteknights, Reading RG6 6AP, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. G.","family":"Coldham","sequence":"additional","affiliation":[{"name":"Department for Food and Environmental Safety, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. J.","family":"Salguero","sequence":"additional","affiliation":[{"name":"Pathology Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. A.","family":"Cooley","sequence":"additional","affiliation":[{"name":"Molecular Pathogenesis Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. R.","family":"Newell","sequence":"additional","affiliation":[{"name":"Centre for Epidemiology and Risk Analysis, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. A.","family":"Rastall","sequence":"additional","affiliation":[{"name":"The University of Reading, Department of Food and Nutritional Sciences, Whiteknights, Reading RG6 6AP, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. R.","family":"Gibson","sequence":"additional","affiliation":[{"name":"The University of Reading, Department of Food and Nutritional Sciences, Whiteknights, Reading RG6 6AP, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Woodward","sequence":"additional","affiliation":[{"name":"Department for Food and Environmental Safety, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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