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Therefore, identifying genes required for its survival in water could help prevent Legionnaires' disease outbreaks. In the present study, we investigate the role of the sigma factor RpoS in promoting survival in water, where\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            experiences severe nutrient deprivation. The\n            <jats:italic>rpoS<\/jats:italic>\n            mutant showed a strong survival defect compared to the wild-type strain in defined water medium. The transcriptome of the\n            <jats:italic>rpoS<\/jats:italic>\n            mutant during exposure to water revealed that RpoS represses genes associated with replication, translation, and transcription, suggesting that the mutant fails to shut down major metabolic programs. In addition, the\n            <jats:italic>rpoS<\/jats:italic>\n            mutant is transcriptionally more active than the wild-type strain after water exposure. This could be explained by a misregulation of the stringent response in the\n            <jats:italic>rpoS<\/jats:italic>\n            mutant. Indeed, the\n            <jats:italic>rpoS<\/jats:italic>\n            mutant shows an increased expression of\n            <jats:italic>spoT<\/jats:italic>\n            and a corresponding decrease in the level of (p)ppGpp, which is due to the presence of a negative feedback loop between RpoS and SpoT. Therefore, the lack of RpoS causes an aberrant regulation of the stringent response, which prevents the induction of a successful response to starvation.\n          <\/jats:p>","DOI":"10.1128\/aem.03132-14","type":"journal-article","created":{"date-parts":[[2014,11,22]],"date-time":"2014-11-22T04:40:10Z","timestamp":1416631210000},"page":"918-928","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["A Regulatory Feedback Loop between RpoS and SpoT Supports the Survival of Legionella pneumophila in Water"],"prefix":"10.1128","volume":"81","author":[{"given":"Hana","family":"Trigui","sequence":"first","affiliation":[{"name":"Department of Natural Resource Sciences, Faculty of Agricultural and Environmental Sciences, McGill University, Sainte-Anne-de-Bellevue, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Dudyk","sequence":"additional","affiliation":[{"name":"Department of Natural Resource Sciences, Faculty of Agricultural and Environmental Sciences, McGill University, Sainte-Anne-de-Bellevue, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinrok","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Seoul National University, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong-In","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Seoul National University, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastien P.","family":"Faucher","sequence":"additional","affiliation":[{"name":"Department of Natural Resource Sciences, Faculty of Agricultural and Environmental Sciences, McGill University, Sainte-Anne-de-Bellevue, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.41.1.9-16.1981"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.53.8.1775-1779.1987"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(96)10041-X"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.7.2326-2330.1992"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.51.2.422-424.1986"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.8.2420-2425.1992"},{"key":"e_1_3_3_8_2","first-page":"519","article-title":"Survival of Legionella pneumophila within cysts of Acanthamoeba polyphaga following chlorine exposure","volume":"68","author":"Kilvington S","year":"1990","unstructured":"Kilvington S, Price J. 1990. 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