{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:18:56Z","timestamp":1773879536014,"version":"3.50.1"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            It is well known that copper is a key factor regulating expression of the two forms of methane monooxygenase found in proteobacterial methanotrophs. Of these forms, the cytoplasmic, or soluble, methane monooxygenase (sMMO) is expressed only at low copper concentrations. The membrane-bound, or particulate, methane monooxygenase (pMMO) is constitutively expressed with respect to copper, and such expression increases with increasing copper. Recent findings have shown that copper uptake is mediated by a modified polypeptide, or chalkophore, termed methanobactin. Although methanobactin has high specificity for copper, it can bind other metals, e.g., gold. Here we show that in\n            <jats:named-content content-type=\"genus-species\">Methylosinus trichosporium<\/jats:named-content>\n            OB3b, sMMO is expressed and active in the presence of copper if gold is also simultaneously present. Such expression appears to be due to gold binding to methanobactin produced by\n            <jats:named-content content-type=\"genus-species\">M. trichosporium<\/jats:named-content>\n            OB3b, thereby limiting copper uptake. Such expression and activity, however, was significantly reduced if methanobactin preloaded with copper was also added. Further, quantitative reverse transcriptase PCR (RT-qPCR) of transcripts of genes encoding polypeptides of both forms of MMO and SDS-PAGE results indicate that both sMMO and pMMO can be expressed when copper and gold are present, as gold effectively competes with copper for binding to methanobactin. Such findings suggest that under certain geochemical conditions, both forms of MMO may be expressed and active\n            <jats:italic>in situ<\/jats:italic>\n            . Finally, these findings also suggest strategies whereby field sites can be manipulated to enhance sMMO expression, i.e., through the addition of a metal that can compete with copper for binding to methanobactin.\n          <\/jats:p>","DOI":"10.1128\/aem.03151-14","type":"journal-article","created":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T23:40:10Z","timestamp":1416613210000},"page":"1024-1031","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Competition between Metals for Binding to Methanobactin Enables Expression of Soluble Methane Monooxygenase in the Presence of Copper"],"prefix":"10.1128","volume":"81","author":[{"given":"Bhagyalakshmi","family":"Kalidass","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Farhan","family":"Ul-Haque","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bipin S.","family":"Baral","sequence":"additional","affiliation":[{"name":"Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan A.","family":"DiSpirito","sequence":"additional","affiliation":[{"name":"Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy D.","family":"Semrau","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0385-6380(86)90118-4"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1246929"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1509"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-011-9873-0"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2011.12.044"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2010.00212.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/elsc.200900093"},{"key":"e_1_3_3_9_2","first-page":"659","volume-title":"Climate change 2013: the physical science basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change","author":"Myhre G","year":"2013","unstructured":"Myhre G, Shindell D, Br\u00e9on F-M, Collins W, Fuglestvedt J, Huang J, Koch D, Lamarque J-F, Lee D, Mendoza B, Nakajima T, Robock A, Stephens G, Takemura T, Zhang H. 2013. Anthropogenic and natural radiative forcing, p 659\u2013740. In Stocker TF, Qin D, Plattner G-K, Tignor M, Allen SK, Boschung J, Nauels A, Xia Y, Bex V, Midgley PM (ed), Climate change 2013: the physical science basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, United Kingdom."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsoil.2012.12.014"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2009.00022.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03426-12"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-386905-0.00008-5"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13192"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26061-0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00696456"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-386905-0.00009-7"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26060-0"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1208255109"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01604-06"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-009-1977-9"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.19.5755-5764.2003"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2004.00572.x"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja804747d"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1112921109"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi1014375"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1098322"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2012.02.002"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/ic101965j"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi051815t"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2006.08.017"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01673-13"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-61-2-205"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-386905-0.00017-6"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12150"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00117048"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_3_3_38_2","volume-title":"Surface complexation modeling: hydrous ferric oxide","author":"Dzombak DA","year":"1990","unstructured":"Dzombak DA, Morel FMM. 1990. Surface complexation modeling: hydrous ferric oxide. John Wiley & Sons, New York, NY."},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1021\/es980125s"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2014.09.004"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00132233"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00969-14"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-008-0445-8"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02292-12"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.03151-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.03151-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T15:08:59Z","timestamp":1645628939000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.03151-14"}},"subtitle":[],"editor":[{"given":"F. E.","family":"L\u00f6ffler","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["10.1128\/AEM.03151-14"],"URL":"https:\/\/doi.org\/10.1128\/aem.03151-14","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2]]},"assertion":[{"value":"2014-09-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-11-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-01-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}