{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:24:05Z","timestamp":1791285845777,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2015,1,15]],"date-time":"2015-01-15T00:00:00Z","timestamp":1421280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2015,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The presence of\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    spp. was investigated in domestic ungulates grazing in communal pastures from a mountain area in northern Spain, where 18.3% (17\/93) of cattle were found to be positive by PCR combined with a reverse line blot (PCR\/RLB), whereas sheep (\n                    <jats:italic>n<\/jats:italic>\n                    = 133) or horses (\n                    <jats:italic>n<\/jats:italic>\n                    = 91) were found not to be infected by this pathogen.\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    infection was significantly associated with age, since older animals showed a higher prevalence than heifers and calves. In contrast to other studies,\n                    <jats:named-content content-type=\"genus-species\">B. chomelii<\/jats:named-content>\n                    was the most frequent species found in cattle (14\/17), while\n                    <jats:named-content content-type=\"genus-species\">B. bovis<\/jats:named-content>\n                    was detected in only three animals. Moreover, 18\n                    <jats:named-content content-type=\"genus-species\">B. chomelii<\/jats:named-content>\n                    isolates and one\n                    <jats:named-content content-type=\"genus-species\">B. bovis<\/jats:named-content>\n                    isolate were obtained from nine animals. Afterwards,\n                    <jats:named-content content-type=\"genus-species\">B. chomelii<\/jats:named-content>\n                    isolates were characterized by a multilocus sequence typing (MLST) method which was adapted in this study. This method presented a high discrimination power, identifying nine different sequence types (STs). This characterization also showed the presence of different STs simultaneously in the same host and that STs had switched over time in one of the animals. In addition,\n                    <jats:named-content content-type=\"genus-species\">B. chomelii<\/jats:named-content>\n                    STs seem to group phylogenetically in two different lineages. The only\n                    <jats:named-content content-type=\"genus-species\">B. bovis<\/jats:named-content>\n                    isolate was characterized with a previously described MLST method. This isolate corresponded to a new ST which is located in lineage I, where the\n                    <jats:named-content content-type=\"genus-species\">B. bovis<\/jats:named-content>\n                    strains infecting\n                    <jats:named-content content-type=\"genus-species\">Bos taurus<\/jats:named-content>\n                    subsp.\n                    <jats:named-content content-type=\"genus-species\">taurus<\/jats:named-content>\n                    are grouped. Further studies on the dynamics of\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    infection in cattle and the potential ectoparasites involved in the transmission of this microorganism should be performed, improving knowledge about the interaction of\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    spp. and domestic ungulates.\n                  <\/jats:p>","DOI":"10.1128\/aem.03159-14","type":"journal-article","created":{"date-parts":[[2014,11,8]],"date-time":"2014-11-08T01:58:59Z","timestamp":1415411939000},"page":"623-629","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Bartonella chomelii Is the Most Frequent Species Infecting Cattle Grazing in Communal Mountain Pastures in Spain"],"prefix":"10.1128","volume":"81","author":[{"given":"M. L.","family":"Antequera-G\u00f3mez","sequence":"first","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Lozano-Almendral","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. F.","family":"Barandika","sequence":"additional","affiliation":[{"name":"NEIKER-Instituto Vasco de Investigaci\u00f3n y Desarrollo Agrario, Derio, Vizcaya, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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L.","family":"Garc\u00eda-P\u00e9rez","sequence":"additional","affiliation":[{"name":"NEIKER-Instituto Vasco de Investigaci\u00f3n y Desarrollo Agrario, Derio, Vizcaya, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Gil","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05009-11"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1007\/0-387-29298-5_89","volume-title":"Bergey's manual of systematic bacteriology","author":"Welch DF","year":"2005","unstructured":"Welch DF. 2005. Family II. Bartonellaceae, p 362\u2013370. In Brenner K, Staley G (ed), Bergey's manual of systematic bacteriology. 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