{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T09:22:10Z","timestamp":1782811330741,"version":"3.54.5"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Lantibiotics are ribosomally synthesized, posttranslationally modified antimicrobial peptides. Their biosynthesis genes are usually organized in gene clusters, which are mainly found in Gram-positive bacteria, including pathogenic streptococci. Three highly virulent\n            <jats:named-content content-type=\"genus-species\">Streptococcus suis<\/jats:named-content>\n            serotype 2 strains (98HAH33, 05ZYH33, and SC84) have been shown to contain an 89K pathogenicity island. Here, on these islands, we unveiled and reannotated a putative lantibiotic locus designated\n            <jats:italic>sui<\/jats:italic>\n            which contains a virulence-associated two-component regulator,\n            <jats:italic>suiK-suiR. In silico<\/jats:italic>\n            analysis revealed that the putative lantibiotic modification gene\n            <jats:italic>suiM<\/jats:italic>\n            was interrupted by a 7.9-kb integron and that other biosynthesis-related genes contained various frameshift mutations. By reconstituting the intact\n            <jats:italic>suiM<\/jats:italic>\n            in\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            together with a semi-\n            <jats:italic>in vitro<\/jats:italic>\n            biosynthesis system, a putative lantibiotic named suicin was produced with bactericidal activities against a variety of Gram-positive strains, including pathogenic streptococci and vancomycin-resistant enterococci. Ring topology dissection indicated that the 34-amino-acid lantibiotic contained two methyllanthionine residues and one disulfide bridge, which render suicin in an N-terminal linear and C-terminal globular shape. To confirm the function of\n            <jats:italic>suiK-suiR<\/jats:italic>\n            , SuiR was overexpressed and purified.\n            <jats:italic>In vitro<\/jats:italic>\n            analysis showed that SuiR could specifically bind to the\n            <jats:italic>suiA<\/jats:italic>\n            gene promoter. Its coexpression with\n            <jats:italic>suiK<\/jats:italic>\n            could activate\n            <jats:italic>suiA<\/jats:italic>\n            gene promoter in\n            <jats:named-content content-type=\"genus-species\">Lactococcus lactis<\/jats:named-content>\n            NZ9000. Conclusively, we obtained a novel lantibiotic suicin by restoring its production from the remnant\n            <jats:italic>sui<\/jats:italic>\n            locus and demonstrated that virulence-associated SuiK-SuiR regulates its production.\n          <\/jats:p>","DOI":"10.1128\/aem.03213-13","type":"journal-article","created":{"date-parts":[[2013,11,23]],"date-time":"2013-11-23T02:13:18Z","timestamp":1385172798000},"page":"1062-1071","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Restoration of Bioactive Lantibiotic Suicin from a Remnant\n            <i>lan<\/i>\n            Locus of Pathogenic Streptococcus suis Serotype 2"],"prefix":"10.1128","volume":"80","author":[{"given":"Jian","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"},{"name":"University of Chinese Academy of Sciences, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"},{"name":"University of Chinese Academy of Sciences, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunling","family":"Teng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shutao","family":"Sun","sequence":"additional","affiliation":[{"name":"Core Facility, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1267"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2174\/157016309787581075"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1517\/17425255.2011.573478"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr030105v"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.199110513"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-11-3561"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0543043"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02295.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020660321724"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.2174\/138920312803582997"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00310.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01254-06"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00883-09"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00730-09"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt391"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-679"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01375-09"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030151"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.309.5739.1308a"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000315"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002080"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006072"},{"key":"e_1_3_2_24_2","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"1989","unstructured":"SambrookJFritschEManiatisT. 1989. 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