{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:18:00Z","timestamp":1791296280499,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2016,2,15]],"date-time":"2016-02-15T00:00:00Z","timestamp":1455494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"crossref","award":["2013BAD19B09-2"],"award-info":[{"award-number":["2013BAD19B09-2"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008990","name":"Zhejiang Provincial Department of Science and Technology","doi-asserted-by":"crossref","award":["2014C02003"],"award-info":[{"award-number":["2014C02003"]}],"id":[{"id":"10.13039\/501100008990","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30870068"],"award-info":[{"award-number":["30870068"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n                    is a saprophytic bacterium that thrives in diverse environments and causes listeriosis via ingestion of contaminated food. RsbX, a putative sigma B (\u03c3\n                    <jats:sup>B<\/jats:sup>\n                    ) regulator, is thought to maintain the ready state in the absence of stress and reset the bacterium to the initial state in the poststress stage in\n                    <jats:named-content content-type=\"genus-species\">Bacillus subtilis<\/jats:named-content>\n                    . We wondered whether RsbX is functional in\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    under different stress scenarios. Genetic deletion and complementation of the\n                    <jats:italic>rsbX<\/jats:italic>\n                    gene were combined with survival tests and transcriptional and translational analyses of \u03c3\n                    <jats:sup>B<\/jats:sup>\n                    expression in response to stresses. We found that deletion of\n                    <jats:italic>rsbX<\/jats:italic>\n                    increased survival under secondary stress following recovery of growth after primary stress or following stationary-phase culturing. The \u0394\n                    <jats:italic>rsbX<\/jats:italic>\n                    mutant had higher expression of \u03c3\n                    <jats:sup>B<\/jats:sup>\n                    than its parent strain in the recovery stage following primary sodium stress and in stationary-phase cultures. Apparently, increased \u03c3\n                    <jats:sup>B<\/jats:sup>\n                    expression had contributed to improved survival in the absence of RsbX. There were no significant differences in survival rates or \u03c3\n                    <jats:sup>B<\/jats:sup>\n                    expression levels in response to primary stresses between the\n                    <jats:italic>rsbX<\/jats:italic>\n                    mutant and its parent strain during the exponential phase. Therefore, we provide clear evidence that RsbX is a negative regulator of\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    \u03c3\n                    <jats:sup>B<\/jats:sup>\n                    during the recovery period after a primary stress or in the stationary phase, thus affecting its survival under secondary stress.\n                  <\/jats:p>","DOI":"10.1128\/aem.03218-15","type":"journal-article","created":{"date-parts":[[2015,12,4]],"date-time":"2015-12-04T22:21:18Z","timestamp":1449267678000},"page":"1126-1135","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["To Modulate Survival under Secondary Stress Conditions, Listeria monocytogenes 10403S Employs RsbX To Downregulate \u03c3\n                    <sup>B<\/sup>\n                    Activity in the Poststress Recovery Stage or Stationary Phase"],"prefix":"10.1128","volume":"82","author":[{"given":"Ye","family":"Xia","sequence":"first","affiliation":[{"name":"Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongping","family":"Xin","sequence":"additional","affiliation":[{"name":"Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoliang","family":"Li","sequence":"additional","affiliation":[{"name":"Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihuan","family":"Fang","sequence":"additional","affiliation":[{"name":"Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, 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