{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:28:10Z","timestamp":1791646090973,"version":"4.3.4"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2013,3,15]],"date-time":"2013-03-15T00:00:00Z","timestamp":1363305600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            strains belonging to serotypes 1\/2a and 4b are frequently linked to listeriosis. While\n            <jats:italic>inlA<\/jats:italic>\n            mutations leading to premature stop codons (PMSCs) and attenuated virulence are common in 1\/2a, they are rare in serotype 4b. We observed PMSCs in 35% of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            isolates (\n            <jats:italic>n<\/jats:italic>\n            = 54) recovered from the British Columbia food supply, including serotypes 1\/2a (30%), 1\/2c (100%), and 3a (100%), and a 3-codon deletion (amino acid positions 738 to 740) seen in 57% of 4b isolates from fish-processing facilities. Caco-2 invasion assays showed that two isolates with the deletion were significantly more invasive than EGD-SmR (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001) and were either as (FF19-1) or more (FE13-1) invasive than a clinical control strain (08-5578) (\n            <jats:italic>P<\/jats:italic>\n            = 0.006). To examine whether serotype 1\/2a was more likely to acquire mutations than other serotypes, strains were plated on agar with rifampin, revealing 4b isolates to be significantly more mutable than 1\/2a, 1\/2c, and 3a serotypes (\n            <jats:italic>P<\/jats:italic>\n            = 0.0002). We also examined the ability of 33 strains to adapt to cold temperature following a downshift from 37\u00ef\u00bf\u00bdC to 4\u00ef\u00bf\u00bdC. Overall, three distinct cold-adapting groups (CAG) were observed: 46% were fast (&lt;70 h), 39% were intermediate (70 to 200 h), and 15% were slow (&gt;200 h) adaptors. Intermediate CAG strains (70%) more frequently possessed\n            <jats:italic>inlA<\/jats:italic>\n            PMSCs than did fast (20%) and slow (10%) CAGs; in contrast, 87% of fast adaptors lacked\n            <jats:italic>inlA<\/jats:italic>\n            PMSCs. In conclusion, we report food chain-derived 1\/2a and 4b serotypes with a 3-codon deletion possessing invasive behavior and the novel association of\n            <jats:italic>inlA<\/jats:italic>\n            genotypes encoding a full-length InlA with fast cold-adaptation phenotypes.\n          <\/jats:p>","DOI":"10.1128\/aem.03341-12","type":"journal-article","created":{"date-parts":[[2013,1,12]],"date-time":"2013-01-12T06:58:28Z","timestamp":1357973908000},"page":"1915-1922","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Examination of Food Chain-Derived Listeria monocytogenes Strains of Different Serotypes Reveals Considerable Diversity in\n            <i>inlA<\/i>\n            Genotypes, Mutability, and Adaptation to Cold Temperatures"],"prefix":"10.1128","volume":"79","author":[{"given":"Jovana","family":"Kovacevic","sequence":"first","affiliation":[{"name":"Food, Nutrition and Health Program, Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Arguedas-Villa","sequence":"additional","affiliation":[{"name":"Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Wozniak","sequence":"additional","affiliation":[{"name":"Food, Nutrition and Health Program, Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, Canada"},{"name":"Department of Epizootiology and Clinic of Bird and Exotic Animals, The Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taurai","family":"Tasara","sequence":"additional","affiliation":[{"name":"Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin J.","family":"Allen","sequence":"additional","affiliation":[{"name":"Food, Nutrition and Health Program, Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0505.990502"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1701.P11101"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.011"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(03)00326-X"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.55.3.476-511.1991"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.2.345"},{"key":"e_1_3_3_8_2","unstructured":"WeatherillS. 2009 posting date. 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