{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:25:41Z","timestamp":1791487541350,"version":"4.3.4"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2016,5,15]],"date-time":"2016-05-15T00:00:00Z","timestamp":1463270400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying\n            <jats:named-content content-type=\"genus-species\">Toxocara<\/jats:named-content>\n            spp. and\n            <jats:named-content content-type=\"genus-species\">Echinococcus multilocularis<\/jats:named-content>\n            , two predominant zoonotic helminths circulating in European carnivores, in feces may help to better target measures for prevention. A rapid, sensitive, and one-step quantitative PCR (qPCR) allowing detection of\n            <jats:named-content content-type=\"genus-species\">E. multilocularis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Toxocara<\/jats:named-content>\n            spp. was developed in the present study, combined with a host fecal test based on the identification of three carnivores (red fox, dog, and cat) involved in the life cycles of these parasites. A total of 68 coprosamples were collected from identified specimens from\n            <jats:named-content content-type=\"genus-species\">Vulpes vulpes<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Canis lupus familiaris<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Canis lupus<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Felis silvestris catus<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Meles meles<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Martes foina<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Martes martes<\/jats:named-content>\n            . With DNA coprosamples, real-time PCR was performed in duplex with a qPCR inhibitor control specifically designed for this study. All the coprosample host identifications were confirmed by qPCR combined with sequencing, and parasites were detected and confirmed (\n            <jats:named-content content-type=\"genus-species\">E. multilocularis<\/jats:named-content>\n            in red foxes and\n            <jats:named-content content-type=\"genus-species\">Toxocara cati<\/jats:named-content>\n            in cats; 16% of samples presented inhibition). By combining parasite detection and quantification, the host fecal test, and a new qPCR inhibitor control, we created a technique with a high sensitivity that may considerably improve environmental studies of pathogens.\n          <\/jats:p>","DOI":"10.1128\/aem.03467-15","type":"journal-article","created":{"date-parts":[[2016,3,12]],"date-time":"2016-03-12T04:03:55Z","timestamp":1457755435000},"page":"2950-2958","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis"],"prefix":"10.1128","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2054-1619","authenticated-orcid":false,"given":"Jenny","family":"Knapp","sequence":"first","affiliation":[{"name":"Chrono-environnement Laboratory, UMR UBFC\/CNRS 6249 aff. INRA, University of Bourgogne Franche-Comt\u00e9, Besan\u00e7on, France"},{"name":"Department of Parasitology-Mycology, University Hospital of Besan\u00e7on, Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9rald","family":"Umhang","sequence":"additional","affiliation":[{"name":"ANSES Nancy Laboratory for Rabies and Wildlife, National Reference Laboratory for Echinococcus spp., Wildlife Surveillance and Eco-Epidemiology Unit, Technopole Agricole et V\u00e9t\u00e9rinaire, Malz\u00e9ville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Lazarine","family":"Poulle","sequence":"additional","affiliation":[{"name":"University of Reims Champagne-Ardenne, SFR Cap-Sant\u00e9, EA3800-PROTAL, UFR de M\u00e9decine, Reims, and CERFE, Boult-aux-Bois, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Millon","sequence":"additional","affiliation":[{"name":"Chrono-environnement Laboratory, UMR UBFC\/CNRS 6249 aff. INRA, University of Bourgogne Franche-Comt\u00e9, Besan\u00e7on, France"},{"name":"Department of Parasitology-Mycology, University Hospital of Besan\u00e7on, Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000722"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1806.111420"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1306.061074"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268811001221"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.07.014"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.07.030"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.08.012"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.06.006"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000081075"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/lt.22299"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.12.034"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-011-2536-8"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.12.035"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2014.07.003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.97.10071467"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.2174\/187153012799278956"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/acprof:oso\/9780199558520.001.0001"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/jzo.12000"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294x.2000.01037.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-009-1629-0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-288"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-011-2272-0"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.12.023"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.07.002"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01240-10"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_3_2_28_2","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall TA","year":"1999","unstructured":"Hall TA. 1999. 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