{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:24:30Z","timestamp":1790630670760,"version":"4.1.0"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2014,2,15]],"date-time":"2014-02-15T00:00:00Z","timestamp":1392422400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Consumption of raw or thermally inadequately treated fishery products represents a public health risk, with the possibility of propagation of live<jats:named-content content-type=\"genus-species\">Anisakis<\/jats:named-content>larvae, the causative agent of the zoonotic disease anisakidosis, or anisakiasis. We investigated the population dynamics of<jats:named-content content-type=\"genus-species\">Anisakis<\/jats:named-content>spp. in commercially important fish\u2014anchovies (<jats:named-content content-type=\"genus-species\">Anisakis<\/jats:named-content>), sardines (<jats:named-content content-type=\"genus-species\">Sardina pilchardus<\/jats:named-content>), European hake (<jats:named-content content-type=\"genus-species\">Merluccius merluccius<\/jats:named-content>), whiting (<jats:named-content content-type=\"genus-species\">Merlangius merlangus<\/jats:named-content>), chub mackerel (<jats:named-content content-type=\"genus-species\">Scomber japonicus<\/jats:named-content>), and Atlantic bluefin tuna (<jats:named-content content-type=\"genus-species\">Thunnus thynnus<\/jats:named-content>)\u2014captured in the main Adriatic Sea fishing ground. We observed a significant difference in the numbers of parasite larvae (1 to 32) in individual hosts and between species, with most fish showing high or very high<jats:named-content content-type=\"genus-species\">Anisakis<\/jats:named-content>population indices. Phylogenetic analysis confirmed that commercial fish in the Adriatic Sea are parasitized by<jats:named-content content-type=\"genus-species\">Anisakis pegreffii<\/jats:named-content>(95.95%) and<jats:named-content content-type=\"genus-species\">Anisakis simplex<\/jats:named-content><jats:named-content content-type=\"genus-species\">sensu stricto<\/jats:named-content>(4.05%). The genetic structure of<jats:named-content content-type=\"genus-species\">A. pegreffii<\/jats:named-content>in demersal, pelagic, and top predator hosts was unstructured, and the highest frequency of haplotype sharing (<jats:italic>n<\/jats:italic>= 10) was between demersal and pelagic fish.<\/jats:p>","DOI":"10.1128\/aem.03561-13","type":"journal-article","created":{"date-parts":[[2013,12,7]],"date-time":"2013-12-07T04:54:59Z","timestamp":1386392099000},"page":"1281-1290","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":79,"title":["Ecology and Genetic Structure of Zoonotic Anisakis spp. from Adriatic Commercial Fish Species"],"prefix":"10.1128","volume":"80","author":[{"given":"Ivona","family":"Mladineo","sequence":"first","affiliation":[{"name":"Institute of Oceanography and Fisheries, Laboratory of Aquaculture, Split, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vedran","family":"Poljak","sequence":"additional","affiliation":[{"name":"Health Ecology Department, Croatian National Institute of Public Health, Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.07.011"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2010.1543"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00043006"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)00202-9"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.03.039"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2011.06.010"},{"key":"e_1_3_3_8_2","first-page":"39","article-title":"Regulation no 1276\/2011 of the European Parliament and of the Council amending annex III to regulation (EC) no 853\/2004 of the European Parliament and of the Council as regards the treatment to kill viable parasites in fishery products for human consumption","volume":"54","author":"European Union","year":"2011","unstructured":"European Union. 9 December 2011. Regulation no 1276\/2011 of the European Parliament and of the Council amending annex III to regulation (EC) no 853\/2004 of the European Parliament and of the Council as regards the treatment to kill viable parasites in fishery products for human consumption. Eur. Lex 54:39. http:\/\/eur-lex.europa.eu\/LexUriServ\/LexUriServ.do?uri=OJ:L:2011:327:0039:0041:EN:PDF.","journal-title":"Eur. Lex"},{"key":"e_1_3_3_9_2","first-page":"91","author":"U.S. Food and Drug Administration","year":"2011","unstructured":"U.S. Food and Drug Administration. 2011. Fish and fishery products hazards and controls guidance, p 91\u201398. U.S. Food and Drug Administration, Washington, DC. http:\/\/www.fda.gov\/downloads\/Food\/GuidanceRegulation\/UCM251970.pdf.","journal-title":"Fish and fishery products hazards and controls guidance"},{"key":"e_1_3_3_10_2","first-page":"44","article-title":"Anisakiasis: is the sushi bar guilty?","volume":"3","author":"Oshima T","year":"1987","unstructured":"OshimaT. 1987. Anisakiasis: is the sushi bar guilty? Trends Parasitol. 3:44\u201348.","journal-title":"Trends Parasitol."},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00012-07"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2007.07.002"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02691.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.10.014"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.07.013"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/656238"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(01)02152-3"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2011.10.001"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)62278-8"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360000202"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00000536"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2008.12.009"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360100389"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-1112.2004.00465.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fishres.2007.09.032"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2012.05.005"},{"key":"e_1_3_3_27_2","volume-title":"The Rapid Alert System for Food and Feed\u2014annual report 2010","author":"Rapid Alert System for Food and Feed","year":"2010","unstructured":"Rapid Alert System for Food and Feed. 2010. The Rapid Alert System for Food and Feed\u2014annual report 2010. Office for Official Publications of the European Communities, Luxembourg. http:\/\/ec.europa.eu\/food\/food\/rapidalert\/docs\/rasff_annual_report_2010_en.pdf."},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.3750\/AIP1976.06.1.08"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.2307\/3284227"},{"key":"e_1_3_3_30_2","volume-title":"Quantitative parasitology 3.0","author":"Reiczigel J","year":"2005","unstructured":"ReiczigelJR\u00f3zsaL. 2005. Quantitative parasitology 3.0. Budapest, Hungary. http:\/\/www.zoologia.hu\/qp\/qp.html."},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2562.1"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-009-9212-8"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.24.4876"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp187"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2010.02847.x"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/14.9.817"},{"key":"e_1_3_3_38_2","first-page":"512","article-title":"Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees","volume":"10","author":"Tamura K","year":"1993","unstructured":"TamuraKNeiM. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 10:512\u2013526.","journal-title":"Mol. Biol. Evol."},{"key":"e_1_3_3_39_2","first-page":"591","article-title":"Signature of ancient population growth in a low-resolution mitochondrial DNA mismatch distribution","volume":"66","author":"Harpending HC","year":"1994","unstructured":"HarpendingHC. 1994. Signature of ancient population growth in a low-resolution mitochondrial DNA mismatch distribution. Hum. Biol. 66:591\u2013600.","journal-title":"Hum. Biol."},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/152.3.1079"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026160"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.8.754"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026036"},{"key":"e_1_3_3_44_2","first-page":"389","article-title":"Anisakis simplex in Adriatic Sea","volume":"105","author":"Mladineo I","year":"2003","unstructured":"MladineoI. 2003. Anisakis simplex in Adriatic Sea. Period. Biol. 105:389\u2013392.","journal-title":"Period. Biol."},{"key":"e_1_3_3_45_2","first-page":"229","article-title":"U\u010destalost nalaza nametnika Anisakis simplex (Anisakidae, Nematoda) u plavoperajnoj tuni (Thunnus thynnus) iz kaveznog uzgoja","volume":"4","author":"\u017dili\u0107 J","year":"2006","unstructured":"\u017dili\u0107JMladineoI. 2006. U\u010destalost nalaza nametnika Anisakis simplex (Anisakidae, Nematoda) u plavoperajnoj tuni (Thunnus thynnus) iz kaveznog uzgoja. Meso 4:229\u2013233.","journal-title":"Meso"},{"key":"e_1_3_3_46_2","first-page":"431","article-title":"A study of Anisakis simplex (Rudolphi 1809) in some economically important teleost fish caught on the Canakkale coast and through the Dardanelles Strait","volume":"4","author":"O\u011fuz MC","year":"2000","unstructured":"O\u011fuzMCGureHOzdemirHOzturkMOSavasY. 2000. A study of Anisakis simplex (Rudolphi 1809) in some economically important teleost fish caught on the Canakkale coast and through the Dardanelles Strait. Turk Parazitol. Derg. 4:431\u2013434.","journal-title":"Turk Parazitol. Derg."},{"key":"e_1_3_3_47_2","first-page":"285","article-title":"Anisakiasis in anchovies (Anisakis) and sardines (Sardina pilchardus) caught along the Adriatic coast","volume":"48","author":"Fioravanti ML","year":"2006","unstructured":"FioravantiMLCaffaraMFlorioDGustinelliAMercerFGradassiMGavaudanSPaoliniAAlessiABiscegliaD. 2006. Anisakiasis in anchovies (Anisakis) and sardines (Sardina pilchardus) caught along the Adriatic coast. Parassitologia 48:285.","journal-title":"Parassitologia"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.09.007"},{"key":"e_1_3_3_49_2","volume-title":"An introduction to marine parasitology","author":"Rohde K","year":"1993","unstructured":"RohdeK. 1993. Ecology of marine parasites. An introduction to marine parasitology. CAB International, London, United Kingdom."},{"key":"e_1_3_3_50_2","first-page":"402","article-title":"Occurrence and spatial distribution of Anisakis sp. in three commercially important pelagic fish stocks from the NE Atlantic, with comments on the significance to consumer safety","volume":"2","author":"Levsen A","year":"2007","unstructured":"LevsenAMidthunE. 2007. Occurrence and spatial distribution of Anisakis sp. in three commercially important pelagic fish stocks from the NE Atlantic, with comments on the significance to consumer safety. Parassitologia 2:402\u2013403.","journal-title":"Parassitologia"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2011.07.026"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2010.09.007"},{"key":"e_1_3_3_53_2","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1093\/oso\/9780198506201.003.0002","volume-title":"The ecology of wildlife diseases","author":"Wilson K","year":"2002","unstructured":"WilsonKBiornstadONDobsonAPMerlerSPoglayenGRandolphSEReadAFSkorpingA. 2002. Heterogeneities in macroparasite infections: patterns and processes, p 6\u201344. In HudsonPJRizzoliAGrenfellBTHeesterbeekHDobsonAP (ed), The ecology of wildlife diseases. Oxford University Press, Oxford, United Kingdom."},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2010.03.019"},{"key":"e_1_3_3_55_2","volume-title":"Jadranska ihtiofauna","author":"Jardas I","year":"1996","unstructured":"JardasI. 1996. Jadranska ihtiofauna. Skolska knjiga, Zagreb, Croatia."},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-004-1261-y"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2009.10.001"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1645\/GE-3504.1"},{"key":"e_1_3_3_59_2","first-page":"89","volume-title":"Molecular identification of Anisakis spp. complex from gastrointestinal tract of stranded cetaceans in Adriatic Sea","author":"Bla\u017eekovi\u0107 K","year":"2012","unstructured":"Bla\u017eekovi\u0107KLepen Plei\u0107I\u00d0uras Gomer\u010di\u0107MGomer\u010di\u0107TMladineoI. 2012. Molecular identification of Anisakis spp. complex from gastrointestinal tract of stranded cetaceans in Adriatic Sea, p 89. Abstr. 26th Eur. Cetacean Soc. Conf. European Cetacean Society, Galway, Ireland."},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mambio.2010.07.002"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.2003.01846.x"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.2307\/j.ctv1nzfgj7"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2109.2011.02828.x"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.03561-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.03561-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T04:53:46Z","timestamp":1716267226000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.03561-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,15]]},"references-count":62,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,2,15]]}},"alternative-id":["10.1128\/AEM.03561-13"],"URL":"https:\/\/doi.org\/10.1128\/aem.03561-13","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,15]]},"assertion":[{"value":"2013-11-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}