{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T05:14:03Z","timestamp":1690434843049},"reference-count":51,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Recently, artificial gene networks have been developed in synthetic biology to control gene expression and make organisms as controllable as robots. Here, I present an artificial posttranslational gene-silencing system based on the codon usage bias and low tRNA content corresponding to minor codons. I engineered the green fluorescent protein (GFP) gene to inhibit translation indirectly with the lowest-usage codons to monopolize various minor tRNAs (\n            <jats:italic>lgfp<\/jats:italic>\n            ). The expression of\n            <jats:italic>lgfp<\/jats:italic>\n            interfered nonspecifically with the growth of\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>\n            , human HeLa cervical cancer cells, MCF7 breast cancer cells, and HEK293 kidney cells, as well as phage and adenovirus expansion. Furthermore, insertion of\n            <jats:italic>lgfp<\/jats:italic>\n            downstream of a phage response promoter conferred phage resistance on\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            . Such engineered gene silencers could act as components of biological networks capable of functioning with suitable promoters in\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">S. cerevisiae<\/jats:named-content>\n            , and human cells to control gene expression. The results presented here show general suppressor artificial genes for live cells and viruses. This robust system provides a gene expression or cell growth control device for artificially synthesized gene networks.\n          <\/jats:p>","DOI":"10.1128\/aem.03708-14","type":"journal-article","created":{"date-parts":[[2015,1,31]],"date-time":"2015-01-31T04:54:28Z","timestamp":1422680068000},"page":"2544-2553","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Inducible Suppression of Global Translation by Overuse of Rare Codons"],"prefix":"10.1128","volume":"81","author":[{"given":"Hideki","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/35002125"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/35002131"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01257"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402940101"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt986"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07389"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.05.045"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(81)90003-6"},{"key":"e_1_3_3_10_2","first-page":"13","article-title":"Codon usage and tRNA content in unicellular and multicellular organisms","volume":"2","author":"Ikemura T","year":"1985","unstructured":"Ikemura T. 1985. 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