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The only genes detected were\n            <jats:italic>eae<\/jats:italic>\n            and\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2<\/jats:sub>\n            , which were carried by 37.69% (\n            <jats:italic>n<\/jats:italic>\n            = 248) of the isolates. Only\n            <jats:italic>eae<\/jats:italic>\n            was harbored by 26.74% (\n            <jats:italic>n<\/jats:italic>\n            = 176) of the isolates, representing potential atypical EPEC strains, while only\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2<\/jats:sub>\n            was detected in 10.33% (\n            <jats:italic>n<\/jats:italic>\n            = 68) of the isolates, indicating potential STEC strains. Moreover, four isolates were positive for both the\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2<\/jats:sub>\n            and\n            <jats:italic>eae<\/jats:italic>\n            genes, representing potential EHEC strains. The prevalence of VGs (\n            <jats:italic>eae<\/jats:italic>\n            or\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2<\/jats:sub>\n            ) was significantly (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001) higher in the fall season, and multiple genes (\n            <jats:italic>eae<\/jats:italic>\n            plus\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2<\/jats:sub>\n            ) were detected only in fall. Repetitive element palindromic PCR (rep-PCR) fingerprint analysis of 658\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            isolates identified 335 unique fingerprints, with an overall Shannon diversity (\n            <jats:italic>H<\/jats:italic>\n            \u2032) index of 3.653. Diversity varied among seasons over the years, with relatively higher diversity during fall. Multivariate analysis of variance (MANOVA) revealed that the majority of the fingerprints showed a tendency to cluster according to year, season, and month. Taken together, the results indicated that the diversity and population structure of\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            fluctuate on a temporal scale, reflecting the presence of diverse host sources and their behavior over time in the watershed. Furthermore, the occurrence of potentially pathogenic\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            strains in the drinking water intakes highlights the risk to human health associated with direct and indirect consumption of untreated surface water.\n          <\/jats:p>","DOI":"10.1128\/aem.03738-14","type":"journal-article","created":{"date-parts":[[2014,12,30]],"date-time":"2014-12-30T08:55:49Z","timestamp":1419929749000},"page":"1788-1798","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Pathogenic Potential, Genetic Diversity, and Population Structure of Escherichia coli Strains Isolated from a Forest-Dominated Watershed (Comox Lake) in British Columbia, Canada"],"prefix":"10.1128","volume":"81","author":[{"given":"Abhirosh","family":"Chandran","sequence":"first","affiliation":[{"name":"Water and Aquatic Sciences Research Program, Department of Biology, University of Victoria, Victoria, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asit","family":"Mazumder","sequence":"additional","affiliation":[{"name":"Water and Aquatic Sciences Research Program, Department of Biology, University of Victoria, Victoria, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.2166\/wh.2006.024"},{"key":"e_1_3_2_3_2","volume-title":"Guidelines for drinking-water quality","author":"WHO","year":"2004","unstructured":"WHO. 2004. 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