{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T17:17:50Z","timestamp":1763140670953},"reference-count":41,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            taxonomy is perpetually being reshuffled, at both the species and intraspecies levels. Biovar 7 of\n            <jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>\n            was suspended from the\n            <jats:italic>Approved Lists of Bacterial Names<\/jats:italic>\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            classification in 1988, because of unpublished evidence that the reference strain 63\/75 was a mixture of\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            biovars 3 and 5. To formally clarify the situation, all isolates previously identified as\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            bv. 7 in the AHVLA and ANSES strain collections were characterized by classical microbiological and multiple molecular approaches. Among the 14 investigated strains, including strain 63\/75, only four strains, isolated in Kenya, Turkey, and Mongolia, were pure and showed a phenotypic profile in agreement with the former biovar 7, particularly agglutination with both anti-A\/anti-M monospecific sera. These results were strengthened by molecular strategies. Indeed, genus- and species-specific methods allowed confirmation that the four pure strains belonged to the\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            species. The combination of most approaches excluded their affiliation with the recognized biovars (biovars 1 to 6 and 9), while some suggested that they were close to biovar 3.These assays were complemented by phylogenetic and\/or epidemiological methods, such as multilocus sequence analysis (MLSA) and variable-number tandem repeat (VNTR) analysis. The results of this polyphasic investigation allow us to propose the reintroduction of biovar 7 into the\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            classification, with at least three representative strains. Interestingly, the Kenyan strain, sharing the same biovar 7 phenotype, was genetically divergent from other three isolates. These discrepancies illustrate the complexity of\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            taxonomy. This study suggests that worldwide collections could include strains misidentified as\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            bv. 7, and it highlights the need to verify their real taxonomic position.\n          <\/jats:p>","DOI":"10.1128\/aem.03755-13","type":"journal-article","created":{"date-parts":[[2013,12,21]],"date-time":"2013-12-21T06:33:36Z","timestamp":1387607616000},"page":"1570-1579","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Examination of Taxonomic Uncertainties Surrounding Brucella abortus bv. 7 by Phenotypic and Molecular Approaches"],"prefix":"10.1128","volume":"80","author":[{"given":"Bruno","family":"Garin-Bastuji","sequence":"first","affiliation":[{"name":"Paris-Est University-ANSES, Animal Health Laboratory, Bacterial Zoonoses Unit, National Reference Centre for Human Brucellosis, National &amp; EU\/OIE\/FAO Reference Laboratory for Animal Brucellosis, Maisons-Alfort, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginie","family":"Mick","sequence":"additional","affiliation":[{"name":"Paris-Est University-ANSES, Animal Health Laboratory, Bacterial Zoonoses Unit, National Reference Centre for Human Brucellosis, National &amp; EU\/OIE\/FAO Reference Laboratory for Animal Brucellosis, Maisons-Alfort, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilles","family":"Le Carrou","sequence":"additional","affiliation":[{"name":"Paris-Est University-ANSES, Animal Health Laboratory, Bacterial Zoonoses Unit, National Reference Centre for Human Brucellosis, National &amp; EU\/OIE\/FAO Reference Laboratory for Animal Brucellosis, Maisons-Alfort, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastien","family":"Allix","sequence":"additional","affiliation":[{"name":"Paris-Est University-ANSES, Animal Health Laboratory, Bacterial Zoonoses Unit, National Reference Centre for Human Brucellosis, National &amp; EU\/OIE\/FAO Reference Laboratory for Animal Brucellosis, Maisons-Alfort, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorraine L.","family":"Perrett","sequence":"additional","affiliation":[{"name":"FAO\/WHO Collaborating Centre for Brucellosis\/OIE Brucellosis Reference Centre, Animal Health and Veterinary Laboratories Agency, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire E.","family":"Dawson","sequence":"additional","affiliation":[{"name":"FAO\/WHO Collaborating Centre for Brucellosis\/OIE Brucellosis Reference Centre, Animal Health and Veterinary Laboratories Agency, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pauline","family":"Groussaud","sequence":"additional","affiliation":[{"name":"FAO\/WHO Collaborating Centre for Brucellosis\/OIE Brucellosis Reference Centre, Animal Health and Veterinary Laboratories Agency, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma J.","family":"Stubberfield","sequence":"additional","affiliation":[{"name":"FAO\/WHO Collaborating Centre for Brucellosis\/OIE Brucellosis Reference Centre, Animal Health and Veterinary Laboratories Agency, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Koylass","sequence":"additional","affiliation":[{"name":"FAO\/WHO Collaborating Centre for Brucellosis\/OIE Brucellosis Reference Centre, Animal Health and Veterinary Laboratories Agency, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian M.","family":"Whatmore","sequence":"additional","affiliation":[{"name":"FAO\/WHO Collaborating Centre for Brucellosis\/OIE Brucellosis Reference Centre, Animal Health and Veterinary Laboratories Agency, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70382-6"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00269-07"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.65269-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.65356-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06351-11"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.011148-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0684.2008.00314.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.06.013"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.08.009"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07509-11"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00620-10"},{"key":"e_1_3_2_13_2","first-page":"260","article-title":"International Committee on Systematic Bacteriology, Subcommittee on Taxonomy of Brucella","volume":"32","year":"1978","unstructured":"International Committee on Systematic Bacteriology, Subcommittee on Taxonomy of Brucella. 1978. 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