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It is prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are commonly used in disease control, and resistance to several antibiotics has been described in\n            <jats:named-content content-type=\"genus-species\">H. parasuis<\/jats:named-content>\n            . Prediction of\n            <jats:named-content content-type=\"genus-species\">H. parasuis<\/jats:named-content>\n            virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with\n            <jats:named-content content-type=\"genus-species\">H. parasuis<\/jats:named-content>\n            virulence, such as\n            <jats:italic>lsgB<\/jats:italic>\n            and group 1\n            <jats:italic>vtaA<\/jats:italic>\n            , while biofilm growth has been associated with nonvirulent strains. In this study, 86\n            <jats:named-content content-type=\"genus-species\">H. parasuis<\/jats:named-content>\n            nasal isolates from farms that had not had a case of disease for more than 10 years were obtained by sampling piglets at weaning. Isolates were studied by enterobacterial repetitive intergenic consensus PCR and determination of the presence of\n            <jats:italic>lsgB<\/jats:italic>\n            and group 1\n            <jats:italic>vtaA<\/jats:italic>\n            , biofilm formation, inflammatory cell response, and resistance to antibiotics. As part of the diversity encountered, a novel 2,661-bp plasmid, named pJMA-1, bearing the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>ROB-1<\/jats:sub>\n            \u03b2-lactamase was detected in eight colonizing strains. pJMA-1 was shown to share a backbone with other small plasmids described in the\n            <jats:named-content content-type=\"genus-species\">Pasteurellaceae<\/jats:named-content>\n            , to be 100% stable, and to have a lower biological cost than the previously described plasmid pB1000. pJMA-1 was also found in nine\n            <jats:named-content content-type=\"genus-species\">H. parasuis<\/jats:named-content>\n            nasal strains from a separate collection, but it was not detected in isolates from the lesions of animals with Gl\u00e4sser's disease or in nontypeable\n            <jats:named-content content-type=\"genus-species\">Haemophilus influenzae<\/jats:named-content>\n            isolates. Altogether, we show that commensal\n            <jats:named-content content-type=\"genus-species\">H. parasuis<\/jats:named-content>\n            isolates represent a reservoir of \u03b2-lactam resistance genes which can be transferred to pathogens or other bacteria.\n          <\/jats:p>","DOI":"10.1128\/aem.03865-14","type":"journal-article","created":{"date-parts":[[2015,3,7]],"date-time":"2015-03-07T03:22:52Z","timestamp":1425698572000},"page":"3255-3267","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Novel\n            <i>bla<\/i>\n            <sub>ROB-1<\/sub>\n            -Bearing Plasmid Conferring Resistance to \u03b2-Lactams in Haemophilus parasuis Isolates from Healthy Weaning Pigs"],"prefix":"10.1128","volume":"81","author":[{"given":"Javier","family":"Moleres","sequence":"first","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda, CSIC-Universidad P\u00fablica Navarra-Gobierno de 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