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Previous research has investigated the fate of individual sewage microorganisms in seawater but not the entire sewage microbial community. The present study used next-generation sequencing (NGS) to examine how the microbial community in sewage-impacted seawater changes over 48 h when exposed to natural sunlight cycles and marine microbiota. We compared the results from microcosms composed of unfiltered seawater (containing naturally occurring marine microbiota) and filtered seawater (containing no marine microbiota) to investigate the effect of marine microbiota. We also compared the results from microcosms that were exposed to natural sunlight cycles with those from microcosms kept in the dark to investigate the effect of sunlight. The microbial community composition and the relative abundance of operational taxonomic units (OTUs) changed over 48 h in all microcosms. Exposure to sunlight had a significant effect on both community composition and OTU abundance. The effect of marine microbiota, however, was minimal. The proportion of sewage-derived microorganisms present in the microcosms decreased rapidly within 48 h, and the decrease was the most pronounced in the presence of both sunlight and marine microbiota, where the proportion decreased from 85% to 3% of the total microbial community. The results from this study demonstrate the strong effect that sunlight has on microbial community composition, as measured by NGS, and the importance of considering temporal effects in future applications of NGS to identify microbial pollution sources.<\/jats:p>","DOI":"10.1128\/aem.03950-14","type":"journal-article","created":{"date-parts":[[2015,1,10]],"date-time":"2015-01-10T05:00:26Z","timestamp":1420866026000},"page":"2107-2116","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Temporal Stability of the Microbial Community in Sewage-Polluted Seawater Exposed to Natural Sunlight Cycles and Marine Microbiota"],"prefix":"10.1128","volume":"81","author":[{"given":"Lauren M.","family":"Sassoubre","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevan M.","family":"Yamahara","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandria B.","family":"Boehm","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2166\/wh.2003.0007"},{"key":"e_1_3_3_3_2","volume-title":"Office of Water report to Congress: impacts and control of CSOs and SSOs","author":"U.S. Environmental Protection Agency","year":"2004","unstructured":"U.S. Environmental Protection Agency. 2004. 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