{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T01:05:18Z","timestamp":1775955918367,"version":"3.50.1"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"23","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Campylobacter jejuni<\/jats:named-content>\n            continues to be the leading cause of bacterial food-borne illness worldwide, so improvements to current methods used for bacterial detection and disease prevention are needed. We describe here the genome and proteome of\n            <jats:named-content content-type=\"genus-species\">C. jejuni<\/jats:named-content>\n            bacteriophage NCTC 12673 and the exploitation of its receptor-binding protein for specific bacterial detection. Remarkably, the 135-kb\n            <jats:named-content content-type=\"genus-species\">Myoviridae<\/jats:named-content>\n            genome of NCTC 12673 differs greatly from any other proteobacterial phage genome described (including\n            <jats:named-content content-type=\"genus-species\">C. jejuni<\/jats:named-content>\n            phages CP220 and CPt10) and instead shows closest homology to the cyanobacterial T4-related myophages. The phage genome contains 172 putative open reading frames, including 12 homing endonucleases, no visible means of packaging, and a putative\n            <jats:italic>trans<\/jats:italic>\n            -splicing intein. The phage DNA appears to be strongly associated with a protein that interfered with PCR amplification and estimation of the phage genome mass by pulsed-field gel electrophoresis. Identification and analyses of the receptor-binding protein (Gp48) revealed features common to the\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            P22 phage tailspike protein, including the ability to specifically recognize a host organism. Bacteriophage receptor-binding proteins may offer promising alternatives for use in pathogen detection platforms.\n          <\/jats:p>","DOI":"10.1128\/aem.05562-11","type":"journal-article","created":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T00:42:02Z","timestamp":1317429722000},"page":"8265-8271","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Genome and Proteome of Campylobacter jejuni Bacteriophage NCTC 12673"],"prefix":"10.1128","volume":"77","author":[{"given":"Andrew M.","family":"Kropinski","sequence":"first","affiliation":[{"name":"Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 110 Stone Road West, Guelph, Ontario N1G 3W4, Canada"},{"name":"Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Arutyunov","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Alberta Innovates Centre for Carbohydrate Science, University of Alberta, Edmonton, Alberta T6G 2E9, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"Foss","sequence":"additional","affiliation":[{"name":"Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Cunningham","sequence":"additional","affiliation":[{"name":"Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Ding","sequence":"additional","affiliation":[{"name":"Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering, University of Alberta and National Institute for Nanotechnology, Edmonton, Alberta T6G 2V4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrey R.","family":"Pavlov","sequence":"additional","affiliation":[{"name":"Fidelity Systems, 7961 Cessna Avenue, Gaithersburg, Maryland 20879"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Henry","sequence":"additional","affiliation":[{"name":"Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephane","family":"Evoy","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering, University of Alberta and National Institute for Nanotechnology, Edmonton, Alberta T6G 2V4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Kelly","sequence":"additional","affiliation":[{"name":"Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine M.","family":"Szymanski","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Alberta Innovates Centre for Carbohydrate Science, University of Alberta, Edmonton, Alberta T6G 2E9, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"AckermannH.-W.AbedonS. T.. 2000. Bacteriophage names 2000: a compilation of known bacteriophages. 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