{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:05:01Z","timestamp":1790867101027,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,3,15]],"date-time":"2012-03-15T00:00:00Z","timestamp":1331769600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Among the leguminous trees native to Uruguay,\n            <jats:named-content content-type=\"genus-species\">Parapiptadenia rigida<\/jats:named-content>\n            (Angico), a Mimosoideae legume, is one of the most promising species for agroforestry. Like many other legumes, it is able to establish symbiotic associations with rhizobia and belongs to the group known as nitrogen-fixing trees, which are major components of agroforestry systems. Information about rhizobial symbionts for this genus is scarce, and thus, the aim of this work was to identify and characterize rhizobia associated with\n            <jats:named-content content-type=\"genus-species\">P. rigida<\/jats:named-content>\n            . A collection of Angico-nodulating isolates was obtained, and 47 isolates were selected for genetic studies. According to enterobacterial repetitive intergenic consensus PCR patterns and restriction fragment length polymorphism analysis of their\n            <jats:italic>nifH<\/jats:italic>\n            and 16S rRNA genes, the isolates could be grouped into seven genotypes, including the genera\n            <jats:named-content content-type=\"genus-species\">Burkholderia<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Cupriavidus<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Rhizobium<\/jats:named-content>\n            , among which the\n            <jats:named-content content-type=\"genus-species\">Burkholderia<\/jats:named-content>\n            genotypes were the predominant group. Phylogenetic studies of\n            <jats:italic>nifH<\/jats:italic>\n            ,\n            <jats:italic>nodA<\/jats:italic>\n            , and\n            <jats:italic>nodC<\/jats:italic>\n            sequences from the\n            <jats:named-content content-type=\"genus-species\">Burkholderia<\/jats:named-content>\n            and the\n            <jats:named-content content-type=\"genus-species\">Cupriavidus<\/jats:named-content>\n            isolates indicated a close relationship of these genes with those from betaproteobacterial rhizobia (beta-rhizobia) rather than from alphaproteobacterial rhizobia (alpha-rhizobia). In addition, nodulation assays with representative isolates showed that while the\n            <jats:named-content content-type=\"genus-species\">Cupriavidus<\/jats:named-content>\n            isolates were able to effectively nodulate\n            <jats:named-content content-type=\"genus-species\">Mimosa pudica<\/jats:named-content>\n            , the\n            <jats:named-content content-type=\"genus-species\">Burkholderia<\/jats:named-content>\n            isolates produced white and ineffective nodules on this host.\n          <\/jats:p>","DOI":"10.1128\/aem.06215-11","type":"journal-article","created":{"date-parts":[[2012,1,7]],"date-time":"2012-01-07T05:05:01Z","timestamp":1325912701000},"page":"1692-1700","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["New Betaproteobacterial Rhizobium Strains Able To Efficiently Nodulate Parapiptadenia rigida (Benth.) Brenan"],"prefix":"10.1128","volume":"78","author":[{"given":"Cecilia","family":"Taul\u00e9","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Gen\u00f3mica Microbianas, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable, MEC, Unidad Asociada a la Facultad de Ciencias, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Zabaleta","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Gen\u00f3mica Microbianas, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable, MEC, Unidad Asociada a la Facultad de Ciencias, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cintia","family":"Mareque","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Gen\u00f3mica Microbianas, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable, MEC, Unidad Asociada a la Facultad de Ciencias, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal","family":"Platero","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Gen\u00f3mica Microbianas, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable, MEC, Unidad Asociada a la Facultad de Ciencias, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc\u00eda","family":"Sanjurjo","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda, Facultad de Agronom\u00eda, UdelaR, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Sicardi","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda del Suelo, Facultad de Ciencias-CIN, UdelaR, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lillian","family":"Frioni","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda, Facultad de Agronom\u00eda, UdelaR, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Battistoni","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Gen\u00f3mica Microbianas, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable, MEC, Unidad Asociada a la Facultad de Ciencias, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Fabiano","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Gen\u00f3mica Microbianas, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable, MEC, Unidad Asociada a la Facultad de Ciencias, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00160-07"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.2.1198-1206.2006"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2004.09.002"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1111\/j.1365-294X.2009.04458.x","article-title":"Burkholderia species are ancient symbionts of legumes","volume":"19","author":"Bontemps C","year":"2010","unstructured":"BontempsC. 2010. 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