{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T16:15:41Z","timestamp":1780416941960,"version":"3.54.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Environmental\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            strains isolated from a coastal brackish pond (Oyster Pond, Woods Hole, MA) carried a novel filamentous phage, VCY\u03d5, which can exist as a host genome integrative form (IF) and a plasmid-like replicative form (RF). Outside the cell, the phage displays a morphology typical of\n            <jats:named-content content-type=\"genus-species\">Inovirus<\/jats:named-content>\n            , with filamentous particles \u223c1.8 \u03bcm in length and 7 nm in width. Four independent RF isolates had identical genomes, except for 8 single nucleotide polymorphisms clustered in two regions. The overall genome size is 7,103 bp with 11 putative open reading frames organized into three functional modules (replication, structure and assembly, and regulation). VCY\u03d5 shares sequence similarity with other filamentous phages (including cholera disease-associated CTX) in a highly mosaic manner, indicating evolution by horizontal gene transfer and recombination. VCY\u03d5 integrates in the vicinity of the putative translation initiation factor Sui1 in chromosome II of\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            . A screen of 531 closely related host isolates showed that \u223c40% harbored phages, with 27% and 13% carrying the IF and RF, respectively. The relative frequencies of the RF and IF differed among strains isolated from the pond or lagoon of Oyster Pond, suggesting that the host habitat influences intracellular phage biology. The overall high prevalence within the host population shows that filamentous phages can be an important component of the environmental biology of\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/aem.06297-11","type":"journal-article","created":{"date-parts":[[2011,10,22]],"date-time":"2011-10-22T04:07:56Z","timestamp":1319256476000},"page":"28-33","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["High Frequency of a Novel Filamentous Phage, VCY\u03d5, within an Environmental Vibrio cholerae Population"],"prefix":"10.1128","volume":"78","author":[{"given":"Hong","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Boucher","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin F.","family":"Polz","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00335-10"},{"key":"e_1_3_2_3_2","unstructured":"CalendarR. 1988. 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A novel filamentous phage, fs-2, of Vibrio cholerae O139. Microbiology 144(Pt. 7):1901\u20131906.","journal-title":"Microbiology"},{"issue":"2","key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1099\/00221287-144-2-315","article-title":"Characterization and possible functions of a new filamentous bacteriophage from Vibrio cholerae O139","volume":"144","author":"Jouravleva EA","year":"1998","unstructured":"JouravlevaEA McDonaldGA GaronCF Boesman-FinkelsteinM FinkelsteinRA. 1998. Characterization and possible functions of a new filamentous bacteriophage from Vibrio cholerae O139. 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