{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:25:19Z","timestamp":1791343519934,"version":"4.3.1"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Brucellosis is one of the major bacterial zoonoses worldwide. In the past decade, an increasing number of atypical\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            strains and species have been described.\n            <jats:named-content content-type=\"genus-species\">Brucella microti<\/jats:named-content>\n            in particular has attracted attention, because this species not only infects mammalian hosts but also persists in soil. An environmental reservoir may pose a new public health risk, leading to the reemergence of brucellosis. In a polyphasic approach, comprising conventional microbiological techniques and extensive biochemical and molecular techniques, all currently available\n            <jats:named-content content-type=\"genus-species\">Brucella microti<\/jats:named-content>\n            strains were characterized. While differing in their natural habitats and host preferences,\n            <jats:named-content content-type=\"genus-species\">B. microti<\/jats:named-content>\n            isolates were found to possess identical 16S rRNA,\n            <jats:italic>recA<\/jats:italic>\n            ,\n            <jats:italic>omp2a<\/jats:italic>\n            , and\n            <jats:italic>omp2b<\/jats:italic>\n            gene sequences and identical multilocus sequence analysis (MLSA) profiles at 21 different genomic loci. Only highly variable microsatellite markers of multiple-locus variable-number tandem repeat (VNTR) analysis comprising 16 loci (MLVA-16) showed intraspecies discriminatory power. In contrast, biotyping demonstrated striking differences within the genetically homologous species. The majority of the mammalian isolates agglutinated only with monospecific anti-M serum, whereas soil isolates agglutinated with anti-A, anti-M, and anti-R sera. Bacteria isolated from animal sources were lysed by phages F1, F25, Tb, BK2, Iz, and Wb, whereas soil isolates usually were not. Rough strains of environmental origin were lysed only by phage R\/C.\n            <jats:named-content content-type=\"genus-species\">B. microti<\/jats:named-content>\n            exhibited high metabolic activities similar to those of closely related soil organisms, such as\n            <jats:named-content content-type=\"genus-species\">Ochrobactrum<\/jats:named-content>\n            spp. Each strain was tested with 93 different substrates and showed an individual metabolic profile. In summary, the adaptation of\n            <jats:named-content content-type=\"genus-species\">Brucella microti<\/jats:named-content>\n            to a specific habitat or host seems to be a matter of gene regulation rather than a matter of gene configuration.\n          <\/jats:p>","DOI":"10.1128\/aem.06351-11","type":"journal-article","created":{"date-parts":[[2011,12,31]],"date-time":"2011-12-31T01:39:24Z","timestamp":1325295564000},"page":"1534-1543","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Intraspecies Biodiversity of the Genetically Homologous Species Brucella microti"],"prefix":"10.1128","volume":"78","author":[{"given":"Sascha","family":"Al Dahouk","sequence":"first","affiliation":[{"name":"Federal Institute for Risk Assessment, Berlin, Germany"},{"name":"RWTH Aachen University, Department of Internal Medicine III, Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Hofer","sequence":"additional","affiliation":[{"name":"Austrian Agency for Health and Food Safety, M\u00f6dling, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Tomaso","sequence":"additional","affiliation":[{"name":"Friedrich Loeffler Institute, Institute of Bacterial Infections and Zoonoses, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Vergnaud","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Sud, Institut de G\u00e9n\u00e9tique et Microbiologie, UMR 8621, Orsay, France"},{"name":"Centre National de la Recherche Scientifique (CNRS), Orsay, France"},{"name":"Mission pour la Recherche et l'Innovation Scientifique (DGA\/MRIS), Bagneux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Le Fl\u00e8che","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Sud, Institut de G\u00e9n\u00e9tique et Microbiologie, UMR 8621, Orsay, France"},{"name":"Centre National de la Recherche Scientifique (CNRS), Orsay, France"},{"name":"Centre d'Etudes du Bouchet, Division of Analytical Microbiology, Vert le Petit, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Cloeckaert","sequence":"additional","affiliation":[{"name":"Institut National de la Recherche Agronomique (INRA), UR1282, Infectiologie Animale et Sant\u00e9 Publique (IASP), Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark S.","family":"Koylass","sequence":"additional","affiliation":[{"name":"Animal Health and Veterinary Laboratories Agency (AHVLA), Department of Bacteriology, New Haw, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian M.","family":"Whatmore","sequence":"additional","affiliation":[{"name":"Animal Health and Veterinary Laboratories Agency (AHVLA), Department of Bacteriology, New Haw, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"N\u00f6ckler","sequence":"additional","affiliation":[{"name":"Federal Institute for Risk Assessment, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger C.","family":"Scholz","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology, Department of Bacteriology, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.12.015"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1111\/j.1439-0450.2005.00898.x","article-title":"Seroprevalence of brucellosis, tularemia, and yersiniosis in wild boars (Sus scrofa) from north-eastern Germany","volume":"52","author":"Al Dahouk S","year":"2005","unstructured":"Al DahoukS. 2005. 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