{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:05:10Z","timestamp":1789578310662,"version":"build-2803163510"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            is the etiological agent of listeriosis, a severe food-borne illness. The population of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            is divided into four lineages (I to IV), and serotype 4b in lineage I has been involved in numerous outbreaks. Several serotype 4b epidemic-associated clonal groups (ECI, -II, and -Ia) have been identified. In this study, we characterized a panel of strains of serotype 4b that produced atypical results with a serotype-specific multiplex PCR and possessed the\n            <jats:italic>lmo0734<\/jats:italic>\n            to\n            <jats:italic>lmo0739<\/jats:italic>\n            gene cassette that had been thought to be specific to lineage II. The cassette was harbored in a genomically syntenic locus in these isolates and in lineage II strains. Three distinct clonal groups (groups 1 to 3) were identified among these isolates based on single-nucleotide polymorphism-based multilocus genotyping (MLGT) and DNA hybridization data. Groups 1 and 2 had MLGT haplotypes previously encountered among clinical isolates and were composed of clinical isolates from multiple states in the United States. In contrast, group 3 consisted of clinical and environmental isolates solely from North Carolina and exhibited a novel haplotype. In addition, all group 3 isolates had DNA that was resistant to MboI, suggesting methylation of adenines at GATC sites. Sequence analysis of the\n            <jats:italic>lmo0734<\/jats:italic>\n            to\n            <jats:italic>lmo0739<\/jats:italic>\n            gene cassette from two strains (group 1 and group 3) revealed that the genes were highly conserved (&gt;99% identity). The data suggest relatively recent horizontal gene transfer from lineage II\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            into\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            serotype 4b and subsequent dissemination among at least three distinct clonal groups of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            serotype 4b, one of which exhibits restrictions in regional distribution.\n          <\/jats:p>","DOI":"10.1128\/aem.06378-11","type":"journal-article","created":{"date-parts":[[2011,12,3]],"date-time":"2011-12-03T23:13:31Z","timestamp":1322954011000},"page":"660-667","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Atypical Listeria monocytogenes Serotype 4b Strains Harboring a Lineage II-Specific Gene Cassette"],"prefix":"10.1128","volume":"78","author":[{"given":"Sangmi","family":"Lee","sequence":"first","affiliation":[{"name":"North Carolina State University, Department of Food, Bioprocessing &amp; Nutrition Sciences, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd J.","family":"Ward","sequence":"additional","affiliation":[{"name":"Bacterial Foodborne Pathogens and Mycology Research Unit, Agricultural Research Service, United States Department of Agriculture, Peoria, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewis M.","family":"Graves","sequence":"additional","affiliation":[{"name":"Enteric Diseases Laboratory Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie A.","family":"Wolf","sequence":"additional","affiliation":[{"name":"NC Laboratory of Public Health, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate","family":"Sperry","sequence":"additional","affiliation":[{"name":"NC Laboratory of Public Health, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin M.","family":"Siletzky","sequence":"additional","affiliation":[{"name":"North Carolina State University, Department of Food, Bioprocessing &amp; Nutrition Sciences, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia","family":"Kathariou","sequence":"additional","affiliation":[{"name":"North Carolina State University, Department of Food, Bioprocessing &amp; Nutrition Sciences, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti553"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03064-09"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg500"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-8-277"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.106.063305"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3819-3822.2004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.2.1072-1083.2004"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01453-06"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.4.2383-2390.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2004.12.002"},{"key":"e_1_3_2_14_2","volume-title":"Proceedings of the 16th International Symposium on Problems of Listeriosis. 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