{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:47:34Z","timestamp":1767962854426,"version":"3.49.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In many bacteria, quorum sensing (QS) systems rely on a signal receptor and a synthase producing\n            <jats:italic>N<\/jats:italic>\n            -acyl-homoserine lactone(s) as the signal molecule(s). In some species, the\n            <jats:italic>rsaL<\/jats:italic>\n            gene, located between the signal receptor and synthase genes, encodes a repressor limiting signal synthase expression and hence signal molecule production. Here we investigate the molecular mechanism of action of the RsaL protein in the plant growth-promoting rhizobacterium\n            <jats:named-content content-type=\"genus-species\">Pseudomonas putida<\/jats:named-content>\n            WCS358 (RsaL\n            <jats:sub>WCS<\/jats:sub>\n            ). In\n            <jats:named-content content-type=\"genus-species\">P. putida<\/jats:named-content>\n            WCS358, RsaL\n            <jats:sub>WCS<\/jats:sub>\n            displayed a strong repressive effect on the promoter of the QS signal synthase gene,\n            <jats:italic>ppuI<\/jats:italic>\n            , while it did not repress the same promoter in\n            <jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa<\/jats:named-content>\n            . DNase I protection assays showed that purified RsaL\n            <jats:sub>WCS<\/jats:sub>\n            specifically binds to\n            <jats:italic>ppuI<\/jats:italic>\n            on a DNA region overlapping the predicted \u03c3\n            <jats:sup>70<\/jats:sup>\n            -binding site, but such protection was observed only at high protein concentrations. Accordingly, electrophoretic mobility shift assays showed that the RsaL\n            <jats:sub>WCS<\/jats:sub>\n            protein was not able to form stable complexes efficiently with a probe encompassing the\n            <jats:italic>ppuI<\/jats:italic>\n            promoter, while it formed stable complexes with the promoter of\n            <jats:italic>lasI<\/jats:italic>\n            , the gene orthologous to\n            <jats:italic>ppuI<\/jats:italic>\n            in\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            . This difference seems to be dictated by the lower dyad symmetry of the RsaL\n            <jats:sub>WCS<\/jats:sub>\n            -binding sequence on the\n            <jats:italic>ppuI<\/jats:italic>\n            promoter relative to that on the\n            <jats:italic>lasI<\/jats:italic>\n            promoter. Comparison of the results obtained\n            <jats:italic>in vivo<\/jats:italic>\n            and\n            <jats:italic>in vitro<\/jats:italic>\n            suggests that RsaL\n            <jats:sub>WCS<\/jats:sub>\n            needs a molecular interactor\/cofactor specific for\n            <jats:named-content content-type=\"genus-species\">P. putida<\/jats:named-content>\n            WCS358 to repress\n            <jats:italic>ppuI<\/jats:italic>\n            transcription. We also demonstrate that RsaL\n            <jats:sub>WCS<\/jats:sub>\n            regulates siderophore-mediated growth limitation of plant pathogens and biofilm formation, two processes relevant for plant growth-promoting activity.\n          <\/jats:p>","DOI":"10.1128\/aem.06442-11","type":"journal-article","created":{"date-parts":[[2011,11,25]],"date-time":"2011-11-25T01:14:00Z","timestamp":1322183640000},"page":"726-734","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Functional Characterization of the Quorum Sensing Regulator RsaL in the Plant-Beneficial Strain Pseudomonas putida WCS358"],"prefix":"10.1128","volume":"78","author":[{"given":"Giordano","family":"Rampioni","sequence":"first","affiliation":[{"name":"Department of Biology, University Roma Tre, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris","family":"Bertani","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology, Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cejoice Ramachandran","family":"Pillai","sequence":"additional","affiliation":[{"name":"Department of Biology, University Roma Tre, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vittorio","family":"Venturi","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology, Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabetta","family":"Zennaro","sequence":"additional","affiliation":[{"name":"Department of Biology, University Roma Tre, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Livia","family":"Leoni","sequence":"additional","affiliation":[{"name":"Department of Biology, University Roma Tre, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.21.6233-6238.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408410902733946"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.syapm.2004.10.005","article-title":"Biofilm formation of Pseudomonas putida IsoF: the role of quorum sensing as assessed by proteomics","volume":"28","author":"Arevalo-Ferro C","year":"2005","unstructured":"Arevalo-FerroC ReilG G\u00f6rgA EberlL RiedelK. 2005. Biofilm formation of Pseudomonas putida IsoF: the role of quorum sensing as assessed by proteomics. Syst. Appl. Microbiol. 28:87\u2013114.","journal-title":"Syst. Appl. Microbiol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2009.0203"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1007\/s10529-010-0347-0","article-title":"Beneficial bacteria of agricultural importance","volume":"32","author":"Babalola OO","year":"2010","unstructured":"BabalolaOO. 2010. Beneficial bacteria of agricultural importance. Biotechnol. Lett. 32:1559\u20131570.","journal-title":"Biotechnol. Lett."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01977588"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/B978-0-12-079870-4.50017-X","volume-title":"Iron chelation in plants and soil microorganisms","author":"Bakker PAHM","year":"1993","unstructured":"BakkerPAHM RaaijmakersJM SchippersB. 1993. Role of iron in the suppression of bacterial plant pathogens by fluorescent pseudomonads, p 269\u2013278. In BartonLL HemmingBC (ed), Iron chelation in plants and soil microorganisms. Academic Press, San Diego, CA."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.9.5493-5502.2004"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(67)80070-6"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.7.2175-2184.1999"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.8.2898-2906.2006"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.3.1198-1209.2001"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.4.1648"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr100109t"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0434.1983.tb00579.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.12.4645"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1129"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.65"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0908760106"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00483-0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00584-1"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.9.5411-5419.2005"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1094\/MPMI-22-12-1514","article-title":"The transcriptional activator rfiA is quorum-sensing regulated by cotranscription with the luxI homolog pcoI and is essential for plant virulence in Pseudomonas corrugata","volume":"22","author":"Licciardello G","year":"2009","unstructured":"LicciardelloG. 2009. The transcriptional activator rfiA is quorum-sensing regulated by cotranscription with the luxI homolog pcoI and is essential for plant virulence in Pseudomonas corrugata. Mol. Plant Microbe Interact. 22:1514\u20131522.","journal-title":"Mol. Plant Microbe Interact."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2010.02316.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1111\/j.1364-3703.2005.00276.x","article-title":"Determinants of Pseudomonas putida WCS358 involved in inducing systemic resistance in plants","volume":"6","author":"Meziane H","year":"2005","unstructured":"MezianeH van der SluisI van LoonLC H\u00f6fteM BakkerPA. 2005. Determinants of Pseudomonas putida WCS358 involved in inducing systemic resistance in plants. Mol. Plant Pathol. 6:177\u2013185.","journal-title":"Mol. Plant Pathol."},{"key":"e_1_3_2_27_2","volume-title":"Experiments in molecular genetics","author":"Miller JH","year":"1972","unstructured":"MillerJH. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00797.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4107"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01552-06"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.2.815-819.2006"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01817.x"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.06029.x"},{"key":"e_1_3_2_34_2","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"1989","unstructured":"SambrookJ FritschEF ManiatisT. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.","edition":"2"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2006.01.036"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.12.6371-6382.2002"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02914-08"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03086-09"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/017780-0"},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1186\/1471-2180-9-125","article-title":"PpoR is a conserved unpaired LuxR solo of Pseudomonas putida which binds N-acyl homoserine lactones","volume":"9","author":"Subramoni S","year":"2009","unstructured":"SubramoniS VenturiV. 2009. PpoR is a conserved unpaired LuxR solo of Pseudomonas putida which binds N-acyl homoserine lactones. BMC Microbiol. 9:125.","journal-title":"BMC Microbiol."},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1094\/MPMI.1997.10.6.716","article-title":"Differential induction of systemic resistance in Arabidopsis by biocontrol bacteria","volume":"10","author":"Van Wees SCM","year":"1997","unstructured":"Van WeesSCM. 1997. Differential induction of systemic resistance in Arabidopsis by biocontrol bacteria. Mol. Plant Microbe Interact. 10:716\u2013724.","journal-title":"Mol. Plant Microbe Interact."},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2005.00012.x"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07890.x"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2009.01.005"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.06442-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.06442-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T19:29:44Z","timestamp":1645644584000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.06442-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["10.1128\/AEM.06442-11"],"URL":"https:\/\/doi.org\/10.1128\/aem.06442-11","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2]]},"assertion":[{"value":"2011-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-11-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-01-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}