{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:23:24Z","timestamp":1790256204483,"version":"4.1.0"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The determination of the success of\n            <jats:italic>in situ<\/jats:italic>\n            bioremediation strategies is complex. By using controlled laboratory conditions, the influence of individual variables, such as U(VI), Cr(VI), and electron donors and acceptors on community structure, dynamics, and the metal-reducing potential can be studied. Triplicate anaerobic, continuous-flow reactors were inoculated with Cr(VI)-contaminated groundwater from the Hanford, WA, 100-H area, amended with lactate, and incubated for 95 days to obtain stable, enriched communities. The reactors were kept anaerobic with N\n            <jats:sub>2<\/jats:sub>\n            gas (9 ml\/min) flushing the headspace and were fed a defined medium amended with 30 mM lactate and 0.05 mM sulfate with a 48-h generation time. The resultant diversity decreased from 63 genera within 12 phyla to 11 bacterial genera (from 3 phyla) and 2 archaeal genera (from 1 phylum). Final communities were dominated by\n            <jats:named-content content-type=\"genus-species\">Pelosinus<\/jats:named-content>\n            spp. and to a lesser degree,\n            <jats:named-content content-type=\"genus-species\">Acetobacterium<\/jats:named-content>\n            spp., with low levels of other organisms, including methanogens. Four new strains of\n            <jats:named-content content-type=\"genus-species\">Pelosinus<\/jats:named-content>\n            were isolated, with 3 strains being capable of Cr(VI) reduction while one also reduced U(VI). Under limited sulfate, it appeared that the sulfate reducers, including\n            <jats:named-content content-type=\"genus-species\">Desulfovibrio<\/jats:named-content>\n            spp., were outcompeted. These results suggest that during times of electron acceptor limitation\n            <jats:italic>in situ<\/jats:italic>\n            , organisms such as\n            <jats:named-content content-type=\"genus-species\">Pelosinus<\/jats:named-content>\n            spp. may outcompete the more-well-studied organisms while maintaining overall metal reduction rates and extents. Finally, lab-scale simulations can test new strategies on a smaller scale while facilitating community member isolation, so that a deeper understanding of community metabolism can be revealed.\n          <\/jats:p>","DOI":"10.1128\/aem.07165-11","type":"journal-article","created":{"date-parts":[[2012,1,21]],"date-time":"2012-01-21T06:00:17Z","timestamp":1327125617000},"page":"2082-2091","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Microbial Community Succession during Lactate Amendment and Electron Acceptor Limitation Reveals a Predominance of Metal-Reducing Pelosinus spp"],"prefix":"10.1128","volume":"78","author":[{"given":"Jennifer J.","family":"Mosher","sequence":"first","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommy J.","family":"Phelps","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mircea","family":"Podar","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Richard A.","family":"Hurt","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James H.","family":"Campbell","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghan M.","family":"Drake","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James G.","family":"Moberly","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher W.","family":"Schadt","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven D.","family":"Brown","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry C.","family":"Hazen","sequence":"additional","affiliation":[{"name":"Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam P.","family":"Arkin","sequence":"additional","affiliation":[{"name":"Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony V.","family":"Palumbo","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris A.","family":"Faybishenko","sequence":"additional","affiliation":[{"name":"Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dwayne A.","family":"Elias","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02881-07"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1093\/nar\/29.2.351","article-title":"The estimation of statistical parameters for local alignment score distributions","volume":"29","author":"Altschul SF","year":"2001","unstructured":"AltschulSF BundschuhR OlsenR HwaT. 2001. 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