{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T07:24:32Z","timestamp":1787815472265,"version":"build-2784847793"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2012,4,15]],"date-time":"2012-04-15T00:00:00Z","timestamp":1334448000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            is responsible for the potentially life-threatening food-borne disease listeriosis. One epidemic-associated clonal group of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            , epidemic clone I (ECI), harbors a Sau3AI-like restriction-modification (RM) system also present in the same genomic region in certain strains of other lineages. In this study, we identified and characterized two other, novel type II RM systems, LmoJ2 and LmoJ3, at this same locus. LmoJ2 and LmoJ3 appeared to recognize GCWGC (W = A or T) and GCNGC, respectively. Both RM systems consisted of genes with GC content below the genome average and were in the same genomic region in strains of different serotypes and lineages, suggesting site-specific horizontal gene transfer. Genomic DNA from the LmoJ2 and LmoJ3 strains grown at various temperatures (4 to 42\u00b0C) was resistant to digestion with restriction enzymes recognizing GCWGC or GCNGC, indicating that the methyltransferases were expressed under these conditions. Phages propagated in an LmoJ2-harboring strain exhibited moderately increased infectivity for this strain at 4 and 8\u00b0C but not at higher temperatures, while phages propagated in an LmoJ3 strain had dramatically increased infectivity for this strain at all temperatures. Among the sequenced\n            <jats:named-content content-type=\"genus-species\">Listeria<\/jats:named-content>\n            phages, lytic phages possessed significantly fewer recognition sites for these RM systems than lysogenic phages, suggesting that in lytic phages sequence content evolved toward reduced susceptibility to such RM systems. The ability of LmoJ2 and LmoJ3 to protect against phages may affect the efficiency of phages as biocontrol agents for\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            strains harboring these RM systems.\n          <\/jats:p>","DOI":"10.1128\/aem.07203-11","type":"journal-article","created":{"date-parts":[[2012,2,11]],"date-time":"2012-02-11T05:30:22Z","timestamp":1328938222000},"page":"2623-2630","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Two Novel Type II Restriction-Modification Systems Occupying Genomically Equivalent Locations on the Chromosomes of Listeria monocytogenes Strains"],"prefix":"10.1128","volume":"78","author":[{"given":"Sangmi","family":"Lee","sequence":"first","affiliation":[{"name":"North Carolina State University, Department of Food, Bioprocessing &amp; Nutrition Sciences, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. J.","family":"Ward","sequence":"additional","affiliation":[{"name":"Bacterial Foodborne Pathogens and Mycology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. M.","family":"Siletzky","sequence":"additional","affiliation":[{"name":"North Carolina State University, Department of Food, Bioprocessing &amp; Nutrition Sciences, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Kathariou","sequence":"additional","affiliation":[{"name":"North Carolina State University, Department of Food, Bioprocessing &amp; Nutrition Sciences, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1128"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2005.08.005"},{"key":"e_1_3_2_5_2","first-page":"1","article-title":"Identification of restriction endonucleases sensitive to 5-cytosin methylation at non-CpG sites, including expanded (CAG)n\/(CTG)n repeats","volume":"6","author":"Castel AL","year":"2011","unstructured":"CastelAL NakamoriM ThorntonCA PearsonCE. 2011. Identification of restriction endonucleases sensitive to 5-cytosin methylation at non-CpG sites, including expanded (CAG)n\/(CTG)n repeats. 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Foodborne outbreaks of listeriosis and epidemic-associated lineages of Listeria monocytogenes, p 243\u2013256. In TorrenceME IsaacsonRE (ed), Microbial food safety in animal agriculture, 1st ed. Iowa State Press, Ames, IA.","edition":"1"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-65.11.1811"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.11.3480-3491.2004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01282-08"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07086-11"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00461-08"},{"key":"e_1_3_2_20_2","first-page":"681","volume-title":"Listeria, listeriosis and food safety","author":"Kornacki JL","year":"2007","unstructured":"KornackiJL GurtlerJB. 2007. Incidence and control of Listeria in food processing facilities, p 681\u2013766. 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