{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:14:18Z","timestamp":1790709258321,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2012,5,15]],"date-time":"2012-05-15T00:00:00Z","timestamp":1337040000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In this study, we focused on evaluating the occurrence of extended-spectrum \u03b2-lactamase (ESBL)-producing\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            in fecal samples of healthy ducks and environmental samples from a duck farm in South China. Duck cloacal swabs and pond water samples were cultivated on MacConkey agar plates supplemented with ceftiofur. Individual colonies were examined for ESBL production. Bacteria identified as\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            were screened for the presence of ESBL and plasmid-borne AmpC genes. The genetic relatedness, plasmid replicon type, and genetic background were determined. Of 245 samples analyzed, 123 had\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            isolates with ceftiofur MICs higher than 8 \u03bcg\/ml (116 [50.4%] from 230 duck samples and 7 [46.7%] from 15 water samples).\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-12<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CMY-2<\/jats:sub>\n            , and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>DHA-1<\/jats:sub>\n            were identified in 108, 5, 9, and 1 isolates, respectively. The most common\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            genes were\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-27<\/jats:sub>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 34),\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-55<\/jats:sub>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 27),\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-24e<\/jats:sub>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 22), and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-105<\/jats:sub>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 20), followed by\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-14a<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-14b<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-24a<\/jats:sub>\n            , and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-24b<\/jats:sub>\n            . Although most of the CTX-M producers had distinct pulsotypes, clonal transmission between duck and water isolates was observed.\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            genes were carried by transferable IncN, IncF, and untypeable plasmids. The novel CTX-M gene\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-105<\/jats:sub>\n            was flanked by two hypothetical protein sequences, partial IS\n            <jats:italic>Ecp1<\/jats:italic>\n            upstream and truncated IS\n            <jats:italic>903D<\/jats:italic>\n            ,\n            <jats:italic>iroN<\/jats:italic>\n            ,\n            <jats:italic>orf1<\/jats:italic>\n            , and a Tn\n            <jats:italic>1721<\/jats:italic>\n            -like element downstream. It is suggested that the horizontal transfer of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            genes mediated by mobile elements and the clonal spread of CTX-M-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            isolates contributed to the dissemination of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            in the duck farm. Our findings highlight the importance of ducks for the dissemination of transferable antibiotic resistance genes into the environment.\n          <\/jats:p>","DOI":"10.1128\/aem.07507-11","type":"journal-article","created":{"date-parts":[[2012,3,10]],"date-time":"2012-03-10T18:51:03Z","timestamp":1331405463000},"page":"3668-3673","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["Characterization of Extended-Spectrum \u03b2-Lactamase Genes Found among Escherichia coli Isolates from Duck and Environmental Samples Obtained on a Duck Farm"],"prefix":"10.1128","volume":"78","author":[{"given":"Junying","family":"Ma","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China"},{"name":"CAS Key Laboratory of Marine Bio-Resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Hua","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luchao","family":"Lv","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyong","family":"Zong","sequence":"additional","affiliation":[{"name":"Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongqing","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZhangLiu","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Ling","family":"Zeng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg256"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"e_1_3_2_5_2","volume-title":"Approved standard","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. 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