{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:25:20Z","timestamp":1791343520489,"version":"4.3.1"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2012,5,15]],"date-time":"2012-05-15T00:00:00Z","timestamp":1337040000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bacterial isolates from frogs were phenotypically identified as\n            <jats:named-content content-type=\"genus-species\">Ochrobactrum anthropi<\/jats:named-content>\n            , but 16S rRNA sequencing showed up to 100% identity with\n            <jats:named-content content-type=\"genus-species\">Brucella inopinata<\/jats:named-content>\n            . Further analysis of\n            <jats:italic>recA<\/jats:italic>\n            ,\n            <jats:italic>omp2a<\/jats:italic>\n            ,\n            <jats:italic>omp2b<\/jats:italic>\n            ,\n            <jats:italic>bcsp31<\/jats:italic>\n            , and IS\n            <jats:italic>711<\/jats:italic>\n            and multilocus sequence analysis (MLSA) verified a close relationship with\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            , suggesting the isolates may actually represent novel members of this growing genus of zoonotic pathogens.\n          <\/jats:p>","DOI":"10.1128\/aem.07509-11","type":"journal-article","created":{"date-parts":[[2012,3,10]],"date-time":"2012-03-10T18:51:03Z","timestamp":1331405463000},"page":"3753-3755","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":97,"title":["Isolation of Potentially Novel Brucella spp. from Frogs"],"prefix":"10.1128","volume":"78","author":[{"given":"Tobias","family":"Eisenberg","sequence":"first","affiliation":[{"name":"Landesbetrieb Hessisches Landeslabor (LHL), Abteilung Veterin\u00e4rmedizin, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Peter","family":"Hamann","sequence":"additional","affiliation":[{"name":"Landesbetrieb Hessisches Landeslabor (LHL), Abteilung Veterin\u00e4rmedizin, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Kaim","sequence":"additional","affiliation":[{"name":"Landesbetrieb Hessisches Landeslabor (LHL), Abteilung Veterin\u00e4rmedizin, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Schlez","sequence":"additional","affiliation":[{"name":"Landesbetrieb Hessisches Landeslabor (LHL), Abteilung Veterin\u00e4rmedizin, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helga","family":"Seeger","sequence":"additional","affiliation":[{"name":"Landesbetrieb Hessisches Landeslabor (LHL), Abteilung Veterin\u00e4rmedizin, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Schauerte","sequence":"additional","affiliation":[{"name":"Zoologischer Garten Frankfurt am Main, Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falk","family":"Melzer","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut (FLI), Institute of Bacterial Infections and Zoonoses, German Reference Center for Brucellosis, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Tomaso","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut (FLI), Institute of Bacterial Infections and Zoonoses, German Reference Center for Brucellosis, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger C.","family":"Scholz","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark S.","family":"Koylass","sequence":"additional","affiliation":[{"name":"OIE\/WHO\/FAO Brucellosis Reference Unit, Department of Bacteriology, Animal Health and Veterinary Laboratories Agency, Addlestone, Surrey, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian M.","family":"Whatmore","sequence":"additional","affiliation":[{"name":"OIE\/WHO\/FAO Brucellosis Reference Unit, Department of Bacteriology, Animal Health and Veterinary Laboratories Agency, Addlestone, Surrey, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Zsch\u00f6ck","sequence":"additional","affiliation":[{"name":"Landesbetrieb Hessisches Landeslabor (LHL), Abteilung Veterin\u00e4rmedizin, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Techniques for the brucellosis laboratory","author":"Alton GG","year":"1988","unstructured":"AltonGG JonesLM AngusRD VergerJM. 1988. 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