{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:16:55Z","timestamp":1791166615826,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            To understand the etiology of exposure-related diseases and to establish standards for reducing the risks associated with working in contaminated environments, the exact nature of the bioaerosol components must be defined. Molecular biology tools were used to evaluate airborne bacterial and, for the first time, archaeal content of dairy barns. Three air samplers were tested in each of the 13 barns sampled. Up to 10\n            <jats:sup>6<\/jats:sup>\n            archaeal and 10\n            <jats:sup>8<\/jats:sup>\n            bacterial 16S rRNA genes per m\n            <jats:sup>3<\/jats:sup>\n            of air were detected. Archaeal methanogens, mainly\n            <jats:named-content content-type=\"genus-species\">Methanobrevibacter<\/jats:named-content>\n            species, were represented.\n            <jats:named-content content-type=\"genus-species\">Saccharopolyspora rectivirgula<\/jats:named-content>\n            , the causative agent of farmer's lung, was quantified to up to 10\n            <jats:sup>7<\/jats:sup>\n            16S rRNA genes per m\n            <jats:sup>3<\/jats:sup>\n            of air. In addition, a wide variety of bacterial agents were present in our air samples within the high airborne bioaerosol concentration range. Despite recommendations regarding hay preservation and baling conditions, farmers still develop an\n            <jats:named-content content-type=\"genus-species\">S. rectivirgula<\/jats:named-content>\n            -specific humoral immune response, suggesting intense and continuous exposure. Our results demonstrate the complexity of bioaerosol components in dairy barns which could play a role in occupational respiratory diseases.\n          <\/jats:p>","DOI":"10.1128\/aem.07661-11","type":"journal-article","created":{"date-parts":[[2012,2,25]],"date-time":"2012-02-25T05:29:55Z","timestamp":1330147795000},"page":"3242-3248","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Characterization of Bioaerosols from Dairy Barns: Reconstructing the Puzzle of Occupational Respiratory Diseases by Using Molecular Approaches"],"prefix":"10.1128","volume":"78","author":[{"given":"Pascale","family":"Blais Lecours","sequence":"first","affiliation":[{"name":"Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec, Qu\u00e9bec, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Veillette","sequence":"additional","affiliation":[{"name":"Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec, Qu\u00e9bec, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Marsolais","sequence":"additional","affiliation":[{"name":"Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec, Qu\u00e9bec, QC, Canada"},{"name":"D\u00e9partement de M\u00e9decine, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Duchaine","sequence":"additional","affiliation":[{"name":"Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec, Qu\u00e9bec, QC, Canada"},{"name":"D\u00e9partement de Biochimie, de Microbiologie et de Bioinformatique, Facult\u00e9 des Sciences et de G\u00e9nie, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Particle size-selective sampling for health-related aerosols","author":"ACGIH","year":"1999","unstructured":"ACGIH. 1999. 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