{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:46:15Z","timestamp":1790739975188,"version":"4.1.0"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have created new complementation constructs for use in\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Neisseria meningitidis<\/jats:named-content>\n            . The constructs contain regions of homology with the chromosome and direct the insertion of a gene of interest into the intergenic region between the genes\n            <jats:italic>iga<\/jats:italic>\n            and\n            <jats:italic>trpB<\/jats:italic>\n            . In order to increase the available options for gene expression in\n            <jats:named-content content-type=\"genus-species\">Neisseria<\/jats:named-content>\n            , we designed the constructs to contain one of three different promoters. One of the constructs contains the isopropyl-\u03b2-\n            <jats:sc>d<\/jats:sc>\n            -thiogalactopyranoside-inducible\n            <jats:italic>lac<\/jats:italic>\n            promoter, which has been widely used in\n            <jats:named-content content-type=\"genus-species\">Neisseria<\/jats:named-content>\n            . We also designed a construct that contains the strong, constitutive promoter from the gonococcal\n            <jats:italic>opaB<\/jats:italic>\n            gene. The third construct contains a tetracycline-inducible promoter, a novel use of this promoter in\n            <jats:named-content content-type=\"genus-species\">Neisseria<\/jats:named-content>\n            . We demonstrate that anhydrotetracycline can be used to induce gene expression in the pathogenic\n            <jats:named-content content-type=\"genus-species\">Neisseria<\/jats:named-content>\n            at very low concentrations and without negatively affecting the growth of the organisms. We use these constructs to complement an arginine auxotrophy in\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            as well as to express a translational fusion of alkaline phosphatase with TraW. TraW is a component of the gonococcal type IV secretion system, and we demonstrate that TraW localizes to the periplasm.\n          <\/jats:p>","DOI":"10.1128\/aem.07871-11","type":"journal-article","created":{"date-parts":[[2012,2,11]],"date-time":"2012-02-11T05:30:22Z","timestamp":1328938222000},"page":"3068-3078","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["New Complementation Constructs for Inducible and Constitutive Gene Expression in Neisseria gonorrhoeae and Neisseria meningitidis"],"prefix":"10.1128","volume":"78","author":[{"given":"Meghan E.","family":"Ramsey","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin\u2014Madison School of Medicine and Public Health, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen T.","family":"Hackett","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin\u2014Madison School of Medicine and Public Health, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaitra","family":"Kotha","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin\u2014Madison School of Medicine and Public Health, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph P.","family":"Dillard","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin\u2014Madison School of Medicine and Public Health, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Abramoff MD","year":"2004","unstructured":"AbramoffMD MagalhaesPJ RamSJ. 2004. Image processing with ImageJ. Biophotonics Int. 11:36\u201342.","journal-title":"Biophotonics Int."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1084\/jem.97.6.797","article-title":"Transformation of type specificity of meningococci: change in heritable type induced by type-specific extracts containing desoxyribonucleic acid","volume":"97","author":"Alexander HE","year":"1953","unstructured":"AlexanderHE RedmanW. 1953. Transformation of type specificity of meningococci: change in heritable type induced by type-specific extracts containing desoxyribonucleic acid. J. Exp. Med. 97:797\u2013806.","journal-title":"J. Exp. Med."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00726-09"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.150.2.633-642.1982"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.1971556.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.151.1.77-82.1982"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402340101"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)00890-5"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.79.4.579-590.1960"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.167.3.1009-1015.1986"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)37738-X"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc04a02s23"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02520.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.29261-0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.58.2.162-210.1994"},{"key":"e_1_3_2_17_2","volume-title":"Immunobiology of Neisseria gonorrhoeae","author":"Goldberg ID","year":"1978","unstructured":"GoldbergID SteinbergVI SiddiquiA HartEJ SchaperD. 1978. Attempts to isolate bacteriophage specific for Neisseria gonorrhoeae. In Immunobiology of Neisseria gonorrhoeae. American Society for Microbiology, Washington, DC."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02173639"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04964.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04521.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.16.4718-4726.2001"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.16.5480-5485.2004"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.6.3009-3013.1999"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-002-0799-6"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.145.3.1365-1373.1981"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.85.6.1274-1279.1963"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1093\/infdis\/138.2.160","article-title":"Phenotypic and epidemiologic correlates of auxotype in Neisseria gonorrhoeae","volume":"138","author":"Knapp JS","year":"1978","unstructured":"KnappJS . 1978. Phenotypic and epidemiologic correlates of auxotype in Neisseria gonorrhoeae. J. Infect. Dis. 138:160\u2013165.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00531-07"},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"7881","DOI":"10.1073\/pnas.79.24.7881","article-title":"Genetic and biochemical analysis of gonococcal IgA1 protease: cloning in Escherichia coli and construction of mutants of gonococci that fail to produce the activity","volume":"79","author":"Koomey JM","year":"1982","unstructured":"KoomeyJM GillRE FalkowS. 1982. Genetic and biochemical analysis of gonococcal IgA1 protease: cloning in Escherichia coli and construction of mutants of gonococci that fail to produce the activity. Proc. Natl. Acad. Sci. U. S. A. 79:7881\u20137885.","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02174444"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/am.28.1.70-76.1974"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00664-1"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4191776.x"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.22.6163-6173.2002"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.5.1600-1609.2001"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.17.5567-5574.1992"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)61676-3"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.3.970-975.1992"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.8.2694-2701.1992"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/154.2.523"},{"key":"e_1_3_2_41_2","volume-title":"A short course in bacterial genetics","author":"Miller JH","year":"1992","unstructured":"MillerJH. 1992. A short course in bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY."},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-145-38025"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197811022991802"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.55.4.931-936.1987"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-198311000-00005"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.24.17290"},{"key":"e_1_3_2_47_2","unstructured":"PachulecE. 2010. The type IV secretion systems of Neisseria gonorrhoeae . Ph.D. thesis. University of Groningen Groningen Netherlands."},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.19.5391-5398.2000"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1701"},{"key":"e_1_3_2_50_2","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1139\/m89-179","article-title":"Biochemical and genetic studies with arginine and proline auxotrophs of Neisseria gonorrhoeae","volume":"35","author":"Picard FJ","year":"1989","unstructured":"PicardFJ DillonJR. 1989. Biochemical and genetic studies with arginine and proline auxotrophs of Neisseria gonorrhoeae. Can. J. Microbiol. 35:1069\u20131075.","journal-title":"Can. J. Microbiol."},{"key":"e_1_3_2_51_2","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/1471-2180-7-66","article-title":"Characterization of the dsDNA prophage sequences in the genome of Neisseria gonorrhoeae and visualization of productive bacteriophage","volume":"7","author":"Piekarowicz A","year":"2007","unstructured":"PiekarowiczA . 2007. Characterization of the dsDNA prophage sequences in the genome of Neisseria gonorrhoeae and visualization of productive bacteriophage. BMC Microbiol. 7:66.","journal-title":"BMC Microbiol."},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01357-09"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05966.x"},{"key":"e_1_3_2_54_2","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"1989","unstructured":"SambrookJ FritschEF ManiatisT. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.","edition":"2"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00810-4"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.1.40-46.1990"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.3.735"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.3.1232-1236.1992"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1001277"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.92.5.1364-1371.1966"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01162-10"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1086\/593336"},{"key":"e_1_3_2_63_2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/0378-1119(83)90228-7","article-title":"Construction and characterization of a new shuttle vector, pLES2, capable of functioning in Escherichia coli and Neisseria gonorrhoeae","volume":"25","author":"Stein DC","year":"1983","unstructured":"SteinDC SilverLE ClarkVL YoungFE. 1983. Construction and characterization of a new shuttle vector, pLES2, capable of functioning in Escherichia coli and Neisseria gonorrhoeae. Gene 25:241\u2013247.","journal-title":"Gene"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00561-7"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210496200"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.134.4.886"},{"key":"e_1_3_2_67_2","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/978-1-61779-346-2_4","article-title":"Generating knock-out and complementation strains of Neisseria meningitidis","volume":"799","author":"van Dam V","year":"2012","unstructured":"van DamV BosMP. 2012. Generating knock-out and complementation strains of Neisseria meningitidis. Methods Mol. Biol. 799:55\u201372.","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.23.6408"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.07871-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.07871-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T19:47:37Z","timestamp":1645645657000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.07871-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5]]},"references-count":67,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1128\/AEM.07871-11"],"URL":"https:\/\/doi.org\/10.1128\/aem.07871-11","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5]]},"assertion":[{"value":"2011-12-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-02-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-04-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}