{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:28:20Z","timestamp":1791019700165,"version":"4.1.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1981,4,1]],"date-time":"1981-04-01T00:00:00Z","timestamp":354931200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1981,4]]},"abstract":"<jats:p>\n            One hundred sixty-one strains of adherent bacteria were isolated under anaerobic conditions from four sites on the rumen epithelial surface of sheep fed hay or a hay-grain ration. Before isolation of bacteria, rumen tissue was washed six times in an anaerobic dilution solution, and viable bacteria suspended in the washings were counted. Calculation indicated that unattached bacteria would have been removed from the tissue by this procedure, but a slow and progressive release of attached bacteria also occurred. Nevertheless, a wide range of characteristic morphological types remained associated with the epithelium as demonstrated by scanning electron microscopy. Most of these types were represented among the isolates. Characterization and presumptive identification of the isolates showed that 95.0% belonged to previously described genera of functionally significant rumen bacteria, including\n            <jats:italic>Butyrivibrio<\/jats:italic>\n            sp. (31.1%),\n            <jats:italic>Bacteroides<\/jats:italic>\n            sp. (22.4%),\n            <jats:italic>Selenomonas ruminantium<\/jats:italic>\n            (9.9%),\n            <jats:italic>Succinivibrio dextrinosolvens<\/jats:italic>\n            (8.7%),\n            <jats:italic>Streptococcus bovis<\/jats:italic>\n            (8.1%),\n            <jats:italic>Propionibacterium<\/jats:italic>\n            sp. (4.3%),\n            <jats:italic>Treponema<\/jats:italic>\n            sp. (3.1%), and\n            <jats:italic>Eubacterium<\/jats:italic>\n            sp.,\n            <jats:italic>Lachnospira multiparus<\/jats:italic>\n            , and\n            <jats:italic>Ruminococcus flavefaciens<\/jats:italic>\n            (2.5% each). Eight isolates (5.0%) were not identified.\n            <jats:italic>L. multiparus<\/jats:italic>\n            was recovered only from hay-fed animals; all other genera were obtained from animals fed either ration. All\n            <jats:italic>S. bovis<\/jats:italic>\n            strains and two strains each of\n            <jats:italic>Bacteroides<\/jats:italic>\n            sp. and\n            <jats:italic>Butyrivibrio<\/jats:italic>\n            sp. were aerotolerant; all other strains were strictly anaerobic. Bacteria representing the gram-positive, facultatively anaerobic flora associated with rumen wall tissue (R. J. Wallace, K.-J. Cheng, D. Dinsdale, and E. R. \u00d8rskov, Nature (London)\n            <jats:bold>279<\/jats:bold>\n            :424-426, 1979) were therefore not recovered by the techniques used; instead a different fraction of the adherent population was isolated. The term \u201cepimural\u201d is proposed to describe the flora associated with the rumen epithelium.\n          <\/jats:p>","DOI":"10.1128\/aem.41.4.1020-1028.1981","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T03:09:14Z","timestamp":1577848154000},"page":"1020-1028","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Isolation and Presumptive Identification of Adherent Epithelial Bacteria (\u201cEpimural\u201d Bacteria) from the Ovine Rumen Wall"],"prefix":"10.1128","volume":"41","author":[{"given":"Lorna J.","family":"Mead","sequence":"first","affiliation":[{"name":"Agricultural Microbiology Section, Department of Dairy and Food Science, University of Saskatchewan, Saskatoon, Canada S7N 0W0"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. A.","family":"Jones","sequence":"additional","affiliation":[{"name":"Agricultural Microbiology Section, Department of Dairy and Food Science, University of Saskatchewan, Saskatoon, Canada S7N 0W0"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.41.4.1020-1028.1981","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.41.4.1020-1028.1981","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:27:24Z","timestamp":1645648044000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.41.4.1020-1028.1981"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1981,4]]}},"alternative-id":["10.1128\/aem.41.4.1020-1028.1981"],"URL":"https:\/\/doi.org\/10.1128\/aem.41.4.1020-1028.1981","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,4]]},"assertion":[{"value":"1981-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}