{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T20:58:37Z","timestamp":1760821117114},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1981,7,1]],"date-time":"1981-07-01T00:00:00Z","timestamp":362793600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1981,7]]},"abstract":"<jats:p>\n            Soil cultures, enrichment cultures, and pure culture isolates produced substantial quantities of salicylic acid from naphthalene in a mineral salts medium containing NH\n            <jats:sub>4<\/jats:sub>\n            Cl as the nitrogen source. However, when KNO\n            <jats:sub>3<\/jats:sub>\n            was substituted for NH\n            <jats:sub>4<\/jats:sub>\n            Cl, these same cultures failed to accumulate detectable quantities of salicylic acid but did turn the medium yellow. When an isolate identified as a\n            <jats:italic>Pseudomonas<\/jats:italic>\n            species was used, viable cell numbers were much greater in the medium containing KNO\n            <jats:sub>3<\/jats:sub>\n            , but up to 94% of the naphthalene was utilized in both media. After 48 h of incubation in a 0.1% naphthalene-mineral salts medium, the cultures containing NH\n            <jats:sub>4<\/jats:sub>\n            Cl showed irregular clumped cells, a pH of 4.7, 42 \u03bcg of salicylic acid per ml, and the production of 4.4 ml of CO\n            <jats:sub>2<\/jats:sub>\n            . Under the same conditions, the cultures in the medium containing KNO\n            <jats:sub>3<\/jats:sub>\n            showed uniform cellular morphology, a pH of 7.3, no salicylic acid, the production of 29.7 ml of CO\n            <jats:sub>2<\/jats:sub>\n            , and a distinct yellow coloration of the medium. The differences between nitrogen sources could not be accounted for by pH alone since results obtained using buffered media were similar. Growth with NH\n            <jats:sub>4<\/jats:sub>\n            NO\n            <jats:sub>3<\/jats:sub>\n            displayed a pattern similar to that obtained when NH\n            <jats:sub>4<\/jats:sub>\n            Cl was used. The yellow coloration in the medium containing KNO\n            <jats:sub>3<\/jats:sub>\n            was apparently due to more than one compound, none of which were 1,2-naphthoquinone or acidic in nature, as suggested by other investigators. Further attempts to identify the yellow compounds by high-pressure liquid chromatography, infrared analysis, and gas chromatography-mass spectrometry have been unsuccessful thus far.\n          <\/jats:p>","DOI":"10.1128\/aem.42.1.74-78.1981","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T03:10:39Z","timestamp":1577848239000},"page":"74-78","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Effect of Nitrogen Source on End Products of Naphthalene Degradation"],"prefix":"10.1128","volume":"42","author":[{"given":"Hazel G.","family":"Aranha","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi 39762"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lewis R.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi 39762"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.42.1.74-78.1981","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.42.1.74-78.1981","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:27:46Z","timestamp":1645648066000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.42.1.74-78.1981"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1981,7]]}},"alternative-id":["10.1128\/aem.42.1.74-78.1981"],"URL":"https:\/\/doi.org\/10.1128\/aem.42.1.74-78.1981","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,7]]},"assertion":[{"value":"1981-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}