{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:28:10Z","timestamp":1790724490145,"version":"4.1.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1982,2,1]],"date-time":"1982-02-01T00:00:00Z","timestamp":381369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1982,2]]},"abstract":"<jats:p>\n            A rapid procedure was devised for detecting on solid media bacteria able to degrade water-insoluble, solid hydrocarbons such as the polycyclic aromatic hydrocarbons phenanthrene, anthracene, and biphenyl. After\n            <jats:italic>Alcaligenes faecalis<\/jats:italic>\n            AFK2 was inoculated on a plate containing mineral salts agar, an ethereal solution of phenanthrene (about 10%, wt\/vol) was sprayed on the surface of the plate, and the plate was incubated at 30\u00b0C for 2 to 3 days. Colonies showing degradation were surrounded with clear zones on the opaque plate. A similar clear zone also was formed around colonies which had been grown on a succinate-mineral salts agar or nutrient agar, followed by spraying of the ethereal solution of phenanthrene and further incubating for 1 day. Other phenanthrene-assimilating bacteria, including\n            <jats:italic>Beijerinckia<\/jats:italic>\n            Bwt and\n            <jats:italic>Pseudomonas<\/jats:italic>\n            SPM64, also formed clear zones on phenanthrene-covered agar plates. This method was applicable to detection of bacteria able to assimilate anthracene, naphthalene, and biphenyl.\n          <\/jats:p>","DOI":"10.1128\/aem.43.2.454-457.1982","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T03:15:03Z","timestamp":1577848503000},"page":"454-457","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":280,"title":["Rapid Screen for Bacteria Degrading Water-Insoluble, Solid Hydrocarbons on Agar Plates"],"prefix":"10.1128","volume":"43","author":[{"given":"Hohzoh","family":"Kiyohara","sequence":"first","affiliation":[{"name":"Department of Applied Chemistry, Okayama University of Science, Okayama 700,  and Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Tokyo 113, 2 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazutaka","family":"Nagao","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry, Okayama University of Science, Okayama 700,  and Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Tokyo 113, 2 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiji","family":"Yana","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry, Okayama University of Science, Okayama 700,  and Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Tokyo 113, 2 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","updated-by":[{"DOI":"10.1128\/aem.43.5.1225-1225.1982","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[1982,5,1]],"date-time":"1982-05-01T00:00:00Z","timestamp":389059200000}}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.43.2.454-457.1982","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.43.2.454-457.1982","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:29:11Z","timestamp":1645648151000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.43.2.454-457.1982"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1982,2]]}},"alternative-id":["10.1128\/aem.43.2.454-457.1982"],"URL":"https:\/\/doi.org\/10.1128\/aem.43.2.454-457.1982","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,2]]},"assertion":[{"value":"1982-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}