{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T12:33:10Z","timestamp":1782736390450,"version":"3.54.5"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1982,8,1]],"date-time":"1982-08-01T00:00:00Z","timestamp":397008000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1982,8]]},"abstract":"<jats:p>\n            A simple procedure, based on the rapid filtration and washing of\n            <jats:italic>Frankia<\/jats:italic>\n            vesicle clusters, was devised for the isolation of\n            <jats:italic>Frankia<\/jats:italic>\n            strains from alder actinorhizal root nodules. Of 46\n            <jats:italic>Alnus incana<\/jats:italic>\n            subsp.\n            <jats:italic>rugosa<\/jats:italic>\n            nodules prepared, 42 yielded isolates. A simple medium containing mineral salts, Casamino Acids, and sodium pyruvate proved to be the most effective for isolation. In general, colonies appeared 6 to 20 days after inoculation. On the basis of hyphal morphology, two distinct types of\n            <jats:italic>Frankia<\/jats:italic>\n            strains were characterized. Randomly selected isolates were tested for infectivity, and all formed root nodules on\n            <jats:italic>A. glutinosa.<\/jats:italic>\n            Because of its simplicity and efficiency, the procedure is an improved method for the study of\n            <jats:italic>Frankia<\/jats:italic>\n            diversity in alder root nodules.\n          <\/jats:p>","DOI":"10.1128\/aem.44.2.461-465.1982","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T03:19:14Z","timestamp":1577848754000},"page":"461-465","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":71,"title":["Isolation of\n            <i>Frankia<\/i>\n            Strains from Alder Actinorhizal Root Nodules"],"prefix":"10.1128","volume":"44","author":[{"given":"David R.","family":"Benson","sequence":"first","affiliation":[{"name":"Microbiology Section, The University of Connecticut, Storrs, Connecticut 06268"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.44.2.461-465.1982","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.44.2.461-465.1982","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:29:55Z","timestamp":1645648195000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.44.2.461-465.1982"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,8]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1982,8]]}},"alternative-id":["10.1128\/aem.44.2.461-465.1982"],"URL":"https:\/\/doi.org\/10.1128\/aem.44.2.461-465.1982","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,8]]},"assertion":[{"value":"1982-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}