{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:04:57Z","timestamp":1787501097073,"version":"build-2736575974"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1983,2,1]],"date-time":"1983-02-01T00:00:00Z","timestamp":412905600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1983,2]]},"abstract":"<jats:p>\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (2492\/pJB4JI) matings with\n                    <jats:italic>Erwinia chrysanthemi<\/jats:italic>\n                    produced kanamycin resistant (Km\n                    <jats:sup>r<\/jats:sup>\n                    ) transconjugants, a majority of which were gentamicin sensitive (Gm\n                    <jats:sup>s<\/jats:sup>\n                    ). A small proportion (about 0.8%) of the Km\n                    <jats:sup>r<\/jats:sup>\n                    Gm\n                    <jats:sup>s<\/jats:sup>\n                    clones were either auxotrophic or failed to catabolize galacturonate (Gtu\n                    <jats:sup>\u2212<\/jats:sup>\n                    ). The R plasmid (pJB4JI) DNA was detected in the parent\n                    <jats:italic>E. coli<\/jats:italic>\n                    strain and in a Km\n                    <jats:sup>r<\/jats:sup>\n                    Gm\n                    <jats:sup>r<\/jats:sup>\n                    transconjugant, but not in Km\n                    <jats:sup>r<\/jats:sup>\n                    Gm\n                    <jats:sup>s<\/jats:sup>\n                    <jats:italic>E. chrysanthemi<\/jats:italic>\n                    strains carrying Tn\n                    <jats:italic>5<\/jats:italic>\n                    -induced mutations. In Hfr crosses, Km\n                    <jats:sup>r<\/jats:sup>\n                    (Tn\n                    <jats:italic>5<\/jats:italic>\n                    ) was found linked with most mutations. A majority (&gt;95%) of prototrophic recombinants were Km\n                    <jats:sup>s<\/jats:sup>\n                    , except for Leu\n                    <jats:sup>+<\/jats:sup>\n                    and Arg\n                    <jats:sup>+<\/jats:sup>\n                    recombinants which were 30 to 50% Km\n                    <jats:sup>s<\/jats:sup>\n                    . Spontaneous revertants were obtained for all markers except\n                    <jats:italic>car, gtu, lys, thr<\/jats:italic>\n                    , and\n                    <jats:italic>trp.<\/jats:italic>\n                    Prototrophic revertants, with the exception of Met\n                    <jats:sup>+<\/jats:sup>\n                    , Leu\n                    <jats:sup>+<\/jats:sup>\n                    , or His\n                    <jats:sup>+<\/jats:sup>\n                    clones, were Km\n                    <jats:sup>s<\/jats:sup>\n                    . We conclude from both genetic and physical data that Tn\n                    <jats:italic>5<\/jats:italic>\n                    transposed from pJB4JI into different sites on the chromosome of\n                    <jats:italic>E. chrysanthemi.<\/jats:italic>\n                  <\/jats:p>","DOI":"10.1128\/aem.45.2.644-650.1983","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T22:23:06Z","timestamp":1577830986000},"page":"644-650","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Tn\n                    <i>5<\/i>\n                    -Induced Mutations in the Enterobacterial Phytopathogen\n                    <i>Erwinia chrysanthemi<\/i>"],"prefix":"10.1128","volume":"45","author":[{"given":"Arun K.","family":"Chatterjee","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerry K.","family":"Thurn","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothy A.","family":"Feese","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.45.2.644-650.1983","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.45.2.644-650.1983","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T15:32:12Z","timestamp":1645630332000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.45.2.644-650.1983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1983,2]]}},"alternative-id":["10.1128\/aem.45.2.644-650.1983"],"URL":"https:\/\/doi.org\/10.1128\/aem.45.2.644-650.1983","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,2]]},"assertion":[{"value":"1983-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}