{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T23:14:54Z","timestamp":1777590894642,"version":"3.51.4"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1985,1,1]],"date-time":"1985-01-01T00:00:00Z","timestamp":473385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1985,1]]},"abstract":"<jats:p>\n            Bacteria from the bovine rumen capable of reducing\n            <jats:italic>trans<\/jats:italic>\n            -aconitate to tricarballylate were enriched in an anaerobic chemostat containing rumen fluid medium and aconitate. After 9 days at a dilution rate of 0.07 h\n            <jats:sup>\u22121<\/jats:sup>\n            , the medium was diluted and plated in an anaerobic glove box. Three types of isolates were obtained from the plates (a crescent-shaped organism, a pleomorphic rod, and a spiral-shaped organism), and all three produced tricarballylate in batch cultures that contained glucose and\n            <jats:italic>trans<\/jats:italic>\n            -aconitate. In glucose-limited chemostats (0.10 h\n            <jats:sup>\u22121<\/jats:sup>\n            ),\n            <jats:italic>trans<\/jats:italic>\n            -aconitate reduction was associated with a decrease in the amount of reduced products formed from glucose. The crescent-shaped organism produced less propionate, the pleomorphic rod produced less ethanol, and the spiral made less succinate and possibly H\n            <jats:sub>2<\/jats:sub>\n            . Aconitate reduction by the pleomorphic rod and the spiral organism was associated with a significant increase in cellular dry matter. Experiments with stock cultures of predominant rumen bacteria indicated that\n            <jats:italic>Selenomonas ruminantium<\/jats:italic>\n            , a species taxonomically similar to the crescent-shaped isolate, was an active reducer of\n            <jats:italic>trans<\/jats:italic>\n            -aconitate. Strains of\n            <jats:italic>Bacteroides ruminicola, Butyrivibrio fibrisolvens<\/jats:italic>\n            , and\n            <jats:italic>Megasphaera elsdenii<\/jats:italic>\n            produced little if any tricarballylate.\n            <jats:italic>Wolinella succinogenes<\/jats:italic>\n            produced some tricarballylate. Based on its stability constant for magnesium (\n            <jats:italic>K<\/jats:italic>\n            <jats:sub>eq<\/jats:sub>\n            = 115), tricarballylate could be a factor in the hypomagnesemia that leads to grass tetany.\n          <\/jats:p>","DOI":"10.1128\/aem.49.1.120-126.1985","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T03:37:49Z","timestamp":1577849869000},"page":"120-126","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Enrichment and Isolation of Rumen Bacteria That Reduce\n            <i>trans<\/i>\n            - Aconitic Acid to Tricarballylic Acid"],"prefix":"10.1128","volume":"49","author":[{"given":"James B.","family":"Russell","sequence":"first","affiliation":[{"name":"Agricultural Research Service, U.S. Department of Agriculture, and Department of Animal Science, Cornell University, Ithaca, New York 14853"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.49.1.120-126.1985","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.49.1.120-126.1985","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:34:53Z","timestamp":1645648493000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.49.1.120-126.1985"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1985,1]]}},"alternative-id":["10.1128\/aem.49.1.120-126.1985"],"URL":"https:\/\/doi.org\/10.1128\/aem.49.1.120-126.1985","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,1]]},"assertion":[{"value":"1985-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}