{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T20:11:09Z","timestamp":1773087069585,"version":"3.50.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1985,5,1]],"date-time":"1985-05-01T00:00:00Z","timestamp":483753600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1985,5]]},"abstract":"\n Nutritional and physical factors affecting the decomposition of [\n 14<\/jats:sup>\n C]lignocellulose prepared from Douglas fir (\n Pseudotsuga menziesii<\/jats:italic>\n ) were examined by incubating the labeled substrate with homogenized surface wood scrapings obtained from a Douglas fir log in a Pacific Northwest stream. Incubations were conducted in distilled water, in stream water collected from four different sources, or in a defined mineral salts solution with or without supplemental N (KNO\n 3<\/jats:sub>\n ). Decomposition rates of [\n 14<\/jats:sup>\n C]lignocellulose, as measured by\n 14<\/jats:sup>\n CO\n 2<\/jats:sub>\n evolution, were greater in each of the four filter-sterilized sources of stream water than in distilled water alone. Decomposition experiments conducted in stream water media with the addition of defined mineral salts demonstrated that [\n 14<\/jats:sup>\n C]cellulose decomposition was stimulated 50% by the addition of either KNO\n 3<\/jats:sub>\n or KH\n 2<\/jats:sub>\n PO\n 4<\/jats:sub>\n \/K\n 2<\/jats:sub>\n HPO\n 4<\/jats:sub>\n and further enhanced (167%) by a combination of both. In contrast, [\n 14<\/jats:sup>\n C]lignin decomposition was stimulated (65%) only by the addition of both N and P. Decomposition of [\n 14<\/jats:sup>\n C]lignocellulose was greatest when supplemental KNO\n 3<\/jats:sub>\n was supplied in concentrations of at least 10.0 mg of N liter\n \u22121<\/jats:sup>\n but not increased further by higher concentrations. The decomposition of [\n 14<\/jats:sup>\n C]lignocellulose increased as the incubation temperature was raised and NO\n 3<\/jats:sub>\n \u22121<\/jats:sup>\n -N supplementation further increased these rates between three-and sevenfold over the range of temperatures examined (5 to 22\u00b0C). Accumulation of NH\n 4<\/jats:sub>\n +<\/jats:sup>\n (2 to 4 mg of N liter\n \u22121<\/jats:sup>\n ) was always observed in culture filtrates of incubations which had been supplemented with KNO\n 3<\/jats:sub>\n , the quantity being independent of NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n concentrations \u2265 10 mg of N liter\n \u22121<\/jats:sup>\n . The role of supplemental NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n in the decomposition of [\n 14<\/jats:sup>\n C]lignocellulose is discussed in relation to wood decomposition and the low concentrations of N found in stream ecosystems of the Pacific Northwest.\n <\/jats:p>","DOI":"10.1128\/aem.49.5.1113-1118.1985","type":"journal-article","created":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T03:41:35Z","timestamp":1577850095000},"page":"1113-1118","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Impact of Nitrogen and Phosphorus on [\n 14<\/sup>\n C]Lignocellulose Decomposition by Stream Wood Microflora"],"prefix":"10.1128","volume":"49","author":[{"given":"Nicholas G.","family":"Aumen","sequence":"first","affiliation":[{"name":"Departments of Microbiology, Soil Science, 2 and Fisheries and Wildlife, 3 Oregon State University, Corvallis, Oregon 97331-3804"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Bottomley","sequence":"additional","affiliation":[{"name":"Departments of Microbiology, Soil Science, 2 and Fisheries and Wildlife, 3 Oregon State University, Corvallis, Oregon 97331-3804"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stan V.","family":"Gregory","sequence":"additional","affiliation":[{"name":"Departments of Microbiology, Soil Science, 2 and Fisheries and Wildlife, 3 Oregon State University, Corvallis, Oregon 97331-3804"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.49.5.1113-1118.1985","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.49.5.1113-1118.1985","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T20:37:00Z","timestamp":1645648620000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.49.5.1113-1118.1985"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1985,5]]}},"alternative-id":["10.1128\/aem.49.5.1113-1118.1985"],"URL":"https:\/\/doi.org\/10.1128\/aem.49.5.1113-1118.1985","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,5]]},"assertion":[{"value":"1985-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}